Rat Interleukin 15,IL-15 ELISA KIT

Rat Interleukin 15,IL-15 ELISA KIT

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中文名稱：

大鼠白介素15(IL-15)酶聯免疫試劑盒
貨號：

CSB-E07453r
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠白介素15(IL-15)酶聯免疫試劑盒（CSB-E07453r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的IL15含量。IL15是一種多效性細胞因子，在免疫系統中發揮重要作用。它通過與其受體結合，激活下游信號通路，促進T細胞、B細胞和NK細胞的增殖和分化。IL15在腫瘤免疫治療中具有潛力，能夠激活抗腫瘤免疫，成為癌癥治療的新靶點。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，炎癥反應、免疫性疾病模型構建、藥物干預效果評估、免疫調控機制研究、感染性疾病模型分析及生物標志物篩選、基礎科研與轉化醫學研究中對IL - 15動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
縮寫：

IL15
Uniprot No.：

P97604
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-101
1:2	Average %	93
	Range %	87-99
1:4	Average %	87
	Range %	82-94
1:8	Average %	100
	Range %	95-104

回收率：

The recovery of rat IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	93	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.511	2.567	2.539	2.383
50	1.773	1.783	1.778	1.622
25	1.106	1.154	1.130	0.974
12.5	0.704	0.725	0.715	0.559
6.25	0.415	0.431	0.423	0.267
3.12	0.287	0.297	0.292	0.136
1.56	0.214	0.231	0.223	0.067
0	0.155	0.157	0.156

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Amentoflavone Mitigates Cyclophosphamide-Induced Pulmonary Toxicity: Involvement of-SIRT-1/Nrf2/Keap1 Axis, JAK-2/STAT-3 Signaling, and Apoptosis MF Balaha,Medicina,2023
Interleukin-15 derived from Guanylin?GC-C-expressing macrophages inhibits fatty acid synthase in adipocytes Akieda-Asai S.et al,Peptides,2017
Effects of acute and chronic resistance exercise training on IL-15 expression in rat skeletal muscle. Zanchi NE et al,Eur J Appl Physio,2010

功能：

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation.
基因功能參考文獻：
1. study indicates that training, skeletal muscle phenotype, and metabolic status all influence the temporal pattern of post-exercise changes in IL-15 expression. PMID: 25522723
2. found that in diabetic rats, resistance training increased muscle and serum IL-15 levels PMID: 24006180
3. results suggested that IL-15-Akt signaling harbored the complex signals for muscle-T cell interaction, the regulatory networks have significant potential for the restriction on IL-1beta inflammatory signaling PMID: 23017227
4. IL-15 is related to organ dysfunction during severe acute pancreatitis, and it functions as a protective factor against the organ injuries. PMID: 20332642
5. The results of this study suggest that the IL-15 signal transduction pathway in neural cells is similar to that in immune cells. PMID: 19747902
6. IL-15 might antagonize some cytokine mediated cell death in islet cells. PMID: 12880684
7. IL-15 produced by metastatic colon carcinoma cells can induce hyperplasia in the mucosa adjacent to colon cancer, thus contributing to angiogenesis and progression of the disease PMID: 14581351
8. Cocultivation of lymphocytes with pancreatic stellate cells and IL-15 inhibited spontaneous lymphocyte apoptosis, and this effect was reduced by an anti-IL-15 antibody PMID: 16002563
9. the decrease of CORT secretion by IL-15 is in part because of (i) the decrease of adenylate cyclase activity and cAMP production and (ii) the inhibition of 3beta-hydroxysteroid dehydrogenase and 11beta-hydroxylase activities in rat ZFR cells. PMID: 16405651
10. Increases in IL-15 mRNA in response to atrophic stimuli may be an attempt to counteract muscle mass loss in skeletal muscles of old animals. PMID: 17135303
11. IL-15 might have an inhibitory effect on the physiologic vascular remodeling processes in closing the ductus arteriosus. PMID: 17667861
12. The changes observed in BAT suggest that IL-15 could be implicated in lipid consumption in this tissue by regulating lipid oxidation and probably thermogenesis, processes mediated by UCP1 and UCP3 and PPARdelta and PPARalpha. PMID: 18239634
13. Preservation of IL-15 signaling by calorie restriction may therefore represent a further mechanism contributing to the anti-aging effect of this dietary intervention in skeletal muscle PMID: 19396981
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亞細胞定位：

Secreted.
蛋白家族：

IL-15/IL-21 family
數據庫鏈接：

KEGG: rno:25670

UniGene: Rn.2490

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.