Rat Interleukin 12,IL-12/P40 ELISA KIT

Rat Interleukin 12,IL-12/P40 ELISA KIT

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中文名稱：

大鼠白介素12(IL-12/P40)酶聯(lián)免疫試劑盒
貨號：

CSB-E07362r
規(guī)格：

96T/48T
價格：

￥3200/￥2500
促銷：
其他：

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引用文獻

產(chǎn)品描述：

大鼠白介素12(IL-12/P40)酶聯(lián)免疫試劑盒（CSB-E07362r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12/P40含量。IL12/P40是白細胞介素-12的β亞基，屬于細胞因子家族，對T細胞和自然殺傷細胞有重要作用。其研究機制涉及與受體的結(jié)合和信號轉(zhuǎn)導，對免疫應答和免疫系統(tǒng)穩(wěn)定至關(guān)重要。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研領(lǐng)域探究感染性疾病、自身免疫疾病或腫瘤微環(huán)境中IL-12/P40的動態(tài)變化，為免疫調(diào)控機制研究、動物疾病模型評估或藥物干預效果分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

IL-12/P40
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	96-106
1:2	Average %	94
	Range %	90-98
1:4	Average %	95
	Range %	90-99
1:8	Average %	89
	Range %	82-94

回收率：

The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	103	97-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.329	2.228	2.279	2.077
1000	1.748	1.647	1.698	1.496
500	1.152	1.131	1.142	0.940
250	0.794	0.783	0.789	0.587
125	0.506	0.495	0.501	0.299
62.5	0.415	0.404	0.410	0.208
31.25	0.308	0.303	0.306	0.104
0	0.202	0.201	0.202

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Early-life bisphenol A exposure causes neuronal pyroptosis in juvenile and adult male rats through the NF-κB/IL-1β/NLRP3/caspase-1 signaling pathway: exploration of age and dose as effective covariates using an in vivo and in silico modeling approach AS Al,Molecular and cellular biochemistry,2024
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.