Rat Insulin-Like 3 (INSL3) ELISA Kit

說明書下載

中文名稱：

大鼠胰島素樣因子3(INSL3)酶聯免疫試劑盒
貨號：

CSB-E15850r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠胰島素樣因子3(INSL3)酶聯免疫試劑盒（CSB-E15850r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的INSL3含量。INSL3是一種胰島素樣激素，主要在性腺組織中表達，與睪丸沉降、生殖細胞存活、骨代謝調控相關。在綿羊中，INSL3基因可能與角的有無有關，研究發現其表達與公羊睪丸和母羊卵巢組織的差異顯著，且存在多個潛在功能位點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，可廣泛應用于生殖生物學領域研究，包括睪丸發育機制探索、內分泌調控網絡解析、生殖相關疾病動物模型評估，以及藥物或環境因素對性腺功能影響的體外實驗分析。科研人員可通過檢測不同樣本中 INSL3 的動態變化，深入探究其在雄性生殖健康中的作用機制，為相關基礎研究提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Insl3 ELISA Kit; Rlf ELISA Kit; Insulin-like 3 ELISA Kit; Leydig insulin-like peptide ELISA Kit; Ley-I-L ELISA Kit; Relaxin-like factor) [Cleaved into: Insulin-like 3 B chain; Insulin-like 3 A chain] ELISA Kit
縮寫：

INSL3
Uniprot No.：

Q9WUK0
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat INSL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	101-109
1:2	Average %	97
	Range %	94-99
1:4	Average %	88
	Range %	83-96
1:8	Average %	93
	Range %	89-98

回收率：

The recovery of rat INSL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	99-105
EDTA plasma (n=4)	92	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	1.978	1.944	1.961	1.759
5	1.436	1.545	1.491	1.289
2.5	1.021	1.006	1.014	0.812
1.25	0.665	0.645	0.655	0.453
0.625	0.423	0.412	0.418	0.216
0.312	0.312	0.325	0.319	0.117
0.156	0.255	0.270	0.263	0.061
0	0.198	0.205	0.202

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.
基因功能參考文獻：
1. The observed postnatal difference in gubernacular malformations between two rate strains is due to different levels of the insl3 receptor differ among strains. PMID: 21633115
2. data suggest that INSL3 signaling in fetal gubernaculum induces morphogenetic programs in male genitalia; investigation of differences in gene expression regulation by INSL3 in gubernaculum of male and female genitalia PMID: 20631401
3. Demonstration of INSL3 as the ligand for LGR8 facilitates understanding of the mechanism of testis descent and allows studies on the role of INSL3 in gonadal and other physiological processes PMID: 12114498
4. Rat leucine-rich repeat-containing G protein-coupled receptor 8 bound INSL3 with high affinity, clearly demonstrating that it is the rat INSL3 receptor PMID: 15956681
5. INSL3/leucine-rich repeat-containing G protein-coupled receptor 8 signaling may be involved in the genesis and/or developmental maturation of renal glomeruli PMID: 15956754
6. Prenatal mono-n-butyl phthlate may inhibit the INSL3 gene expression associated with the transabdominal testicular ascent in fetal rats, thereby causing undescended testis in postnatal offspring PMID: 16338306
7. In utero exposure to flutamide decreases expression levels of Insl3 particularly at 15 days post conception. PMID: 18772241
8. INSL3 evidently traverses the blood-testis barrier to enter the seminiferous compartment, rete testis, and epididymis in sufficient concentration to be able to address the specific INSL3 receptors (RXFP2) on post-meiotic germ cells and in the epididymis. PMID: 19420383
9. Prenatal exposure to diaethylstilbestrol can inhibit transabdominal testicular descent via down-regulating the expression of INSL3, which is induced by down-regulating the expression of SF-1. PMID: 19493424
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亞細胞定位：

Secreted.
蛋白家族：

Insulin family
組織特異性：

Expressed in Leydig cells of the testis, and weakly in the theca interna cells of antral follicles and the corpus luteum of the ovary.
數據庫鏈接：

KEGG: rno:114215

STRING: 10116.ENSRNOP00000025349

UniGene: Rn.48700

Rat Insulin-Like 3 (INSL3) ELISA Kit

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