Rat High density lipoprotein cholesterol, HDL-C ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠高密度脂蛋白膽固醇酶聯(lián)免疫試劑盒（CSB-E14399r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HDL-C含量。HDL - C即高密度脂蛋白膽固醇，它被視作“好膽固醇”，背景上與心血管健康密切相關(guān)，較高水平的HDL - C有助于降低心血管疾病風(fēng)險(xiǎn)。其研究機(jī)制主要是促進(jìn)膽固醇逆向轉(zhuǎn)運(yùn)，將外周組織中的膽固醇轉(zhuǎn)運(yùn)到肝臟進(jìn)行代謝，減少膽固醇沉積。試劑盒檢測(cè)范圍為93.75 ng/mL-6000 ng/mL，適用于動(dòng)脈粥樣硬化發(fā)病機(jī)制研究、脂代謝調(diào)控實(shí)驗(yàn)、藥物干預(yù)效果評(píng)價(jià)等科研領(lǐng)域，可支持動(dòng)物模型構(gòu)建、營(yíng)養(yǎng)學(xué)實(shí)驗(yàn)及心血管相關(guān)分子通路的探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

HDL-C
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

93.75 ng/mL-6000 ng/mL
靈敏度：

23.4 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	87
	Range %	83-89
1:400	Average %	99
	Range %	95-102
1:800	Average %	90
	Range %	85-93
1:1600	Average %	99
	Range %	92-102

回收率：

The recovery of rat HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	107	101-109

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.