Rat High density lipoprotein,HDL ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠高密度脂蛋白(HDL)酶聯(lián)免疫試劑盒（CSB-E11703r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的HDL含量。高密度脂蛋白（HDL）是一種重要的血清脂蛋白，其代謝與重塑過程受到多種分子調(diào)節(jié)。研究顯示，HDL在膽固醇代謝中發(fā)揮關(guān)鍵作用，通過抑制膽固醇在血管壁的蓄積，減輕動脈粥樣硬化。目前，針對HDL代謝與重塑的研究正不斷深入，旨在開發(fā)相關(guān)藥物以更全面評價HDL的抗動脈粥樣硬化作用。試劑盒檢測范圍為78 ng/mL-5000 ng/mL，適用于心血管疾病模型研究、脂代謝機(jī)制探索、藥物篩選或營養(yǎng)干預(yù)評估等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

HDL
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

78 ng/mL-5000 ng/mL
靈敏度：

19.5 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	99
	Range %	96-102
1:400	Average %	103
	Range %	100-107
1:800	Average %	89
	Range %	84-96
1:1600	Average %	96
	Range %	94-97

回收率：

The recovery of rat HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	103	99-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.