Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

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中文名稱：

大鼠粒細胞集落刺激因子(G-CSF)酶聯免疫試劑盒
貨號：

CSB-E07340r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠粒細胞集落刺激因子(G-CSF)酶聯免疫試劑盒（CSB-E07340r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的G-CSF含量。G-CSF即粒細胞集落刺激因子，在造血調控中至關重要。它能特異性地作用于造血干細胞，促進粒細胞前體細胞增殖、分化，增強成熟粒細胞功能。臨床常用于放化療后骨髓抑制等，提升粒細胞數量，降低感染風險，研究聚焦其信號通路及新應用。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，在基礎研究中可用于探索免疫系統調控機制、炎癥相關疾病模型中的G-CSF動態變化，或評估藥物/化合物對中性粒細胞生成的影響。例如在感染性疾病模型、骨髓抑制恢復研究或組織損傷修復實驗中，可通過檢測G-CSF表達水平揭示其生物學功能。試劑盒操作流程標準化，配備預包被96孔板及優化反應體系，適用于實驗室常規批量樣本檢測需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
縮寫：

G-CSF
Uniprot No.：

P48750
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-94
1:2	Average %	101
	Range %	97-104
1:4	Average %	95
	Range %	90-97
1:8	Average %	100
	Range %	95-103

回收率：

The recovery of rat G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	94	92-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.133	2.118	2.126	2.007
1000	1.837	1.776	1.807	1.688
500	1.294	1.282	1.288	1.169
250	0.889	0.878	0.884	0.765
125	0.588	0.607	0.598	0.479
62.5	0.361	0.349	0.355	0.236
31.25	0.222	0.216	0.219	0.100
0	0.118	0.120	0.119

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Gut microbial analysis combined with metabolomics reveal the mechanism of stachyose on blood deficiency syndrome in rats W Zhang,Arabian Journal of Chemistry,2024
Serum, spleen metabolomics and gut microbiota reveals effect of catalpol on blood deficiency syndrome caused by cyclophosphamide and … W Zhang,Frontiers in immunology,2023
Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms Golle L.et al,Sci Rep,2017

功能：

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
基因功能參考文獻：
1. Transplant vasculopathy depends on the simultaneous and complementary additive effects of granulocyte-macrophage colony-stimulating factor and cytokines, which have a continuous "cross talk." PMID: 28550897
2. Mesenteric mesothelial cells express GM-CSF receptor on the plasma membrane; during inflammation they synthesize and secrete GM-CSF that can stimulate GM-CSF receptor expression and mesothelial cell to macrophage-like cell transformation; in this transformation, GM-CSF (together with its receptor and other cytokines) has a major autocrine regulatory role; receptor-ligand internalization is essential for GM-CSF signalling PMID: 27364613
3. The findings that ephrinA1-PE38/GM-CSF had a high efficacy in the activation of the dendritic cells would facilitate the development of in vivo dendritic-cell vaccines for the treatment of gliomas in rats PMID: 25677907
4. Monocyte chemotactic protein-1 and GM-CSF may play an important role in the progression of experimental autoimmune myocarditis PMID: 12071442
5. GM-CSF exerts a neuromodulatory effect and may provide a new link of neuroimmune communication. PMID: 14557677
6. We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. PMID: 14651956
7. GM-CSF may play a significant role in follicular development PMID: 15958400
8. GM-CSF signaling in the central nervous system can regulate energy homeostasis. PMID: 16276414
9. GM-CSF signaling as reflected by PU.1 expression and nuclear binding was restored with recombinant GM-CSF treatment in vitro. PMID: 16877635
10. GM-CSF acts as a neuroprotective protein in the CNS PMID: 17457367
11. Maternal administration of GM-CSF induced fetal lung maturation and this effect may be mediated, at least partly, by an increase in the eNOS expression. PMID: 17478029
12. GM-CSF stimulates neuronal differentiation of adult neural stem cells. PMID: 17953750
13. G-CSF improves and GM-CSF exacerbates early postinfarction LV remodeling in association with modulation of DC infiltration. PMID: 18832728
14. IL-1beta promotes tissue catabolism and destruction, whereas GM-CSF enhances tissue reconstruction PMID: 19022682
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亞細胞定位：

Secreted.
蛋白家族：

GM-CSF family
數據庫鏈接：

KEGG: rno:116630

STRING: 10116.ENSRNOP00000034235

UniGene: Rn.44285

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.