Rat Fibrinogen,Fbg ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠血纖蛋白原(Fbg)酶聯免疫試劑盒（CSB-E08201r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Fbg含量。Fbg即纖維蛋白原，是血漿中的凝血因子。其在凝血過程中起關鍵作用，在凝血酶作用下轉變為纖維蛋白參與血栓形成。研究多圍繞其與血栓性疾病關聯展開，探索抑制其功能或生成的機制，以研發抗血栓藥物。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL，適用于凝血機制研究、肝損傷模型評估或炎癥相關疾病模型的科研場景，例如通過檢測血漿樣本分析凝血功能異常，或通過組織勻漿檢測探究局部炎癥反應中Fbg的調控作用。為心血管、肝臟及免疫相關基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

Fbg
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 μg/mL-20 μg/mL
靈敏度：

0.156 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	91-103
1:200	Average %	90
	Range %	82-94
1:400	Average %	102
	Range %	91-106
1:800	Average %	101
	Range %	91-105

回收率：

The recovery of rat Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	93-104
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.