Rat Erythropoietin,EPO ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述：
大鼠紅細(xì)胞生成素(EPO)酶聯(lián)免疫試劑盒（CSB-E07323r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EPO含量。EPO（促紅細(xì)胞生成素）是一種糖蛋白激素，主要在腎臟合成，控制紅細(xì)胞生成。其研究機(jī)制涉及通過JAK2-STAT5通路促進(jìn)紅細(xì)胞生成，同時(shí)具有神經(jīng)保護(hù)作用，如治療神經(jīng)退行性疾病。EPO在類風(fēng)濕關(guān)節(jié)炎等疾病的研究中，發(fā)現(xiàn)其通過上調(diào)神經(jīng)氨酸酶3（NEU3）表達(dá)，降低滑膜成纖維細(xì)胞的唾液酸化水平，進(jìn)而促進(jìn)疾病進(jìn)展。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于探究貧血發(fā)生機(jī)制、腎臟功能損傷模型、高原低氧適應(yīng)性研究以及促紅細(xì)胞生成類藥物開發(fā)的科研實(shí)驗(yàn)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
EpoErythropoietin ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Rattus norvegicus (Rat)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
0.156 ng/mL-10 ng/mL
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靈敏度：
0.039 ng/mL
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反應(yīng)時(shí)間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Immunology
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-92 | |
| 1:2 | Average % | 95 | |
| Range % | 91-99 | |
| 1:4 | Average % | 96 | |
| Range % | 89-101 | |
| 1:8 | Average % | 94 | |
| Range % | 85-99 | |
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回收率：
| The recovery of rat EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 92-100 | |
| EDTA plasma (n=4) | 95 | 86-99 | |
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標(biāo)準(zhǔn)曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.176 | 2.191 | 2.184 | 2.075 | | | 5 | 1.642 | 1.624 | 1.633 | 1.524 | | | 2.5 | 1.236 | 1.218 | 1.227 | 1.118 | | | 1.25 | 0.842 | 0.859 | 0.851 | 0.742 | | | 0.625 | 0.537 | 0.522 | 0.530 | 0.421 | | | 0.312 | 0.373 | 0.381 | 0.377 | 0.268 | | | 0.156 | 0.154 | 0.162 | 0.158 | 0.049 | | | 0 | 0.108 | 0.110 | 0.109 | | |
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本試劑盒所含材料：
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat EPO antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled EPO antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻(xiàn)
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Restoration of renal hemodynamics and functions by Nigella sativa administration in dinitrophenol-induced hypoxia in rat's animal model
AF Hassan,International journal of health sciences,2024
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Gut microbial analysis combined with metabolomics reveal the mechanism of stachyose on blood deficiency syndrome in rats
W Zhang,Arabian Journal of Chemistry,2024
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Serum, spleen metabolomics and gut microbiota reveals effect of catalpol on blood deficiency syndrome caused by cyclophosphamide and …
W Zhang,Frontiers in immunology,2023
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Combined effects of Rhodiola rosea and caffeine supplementation on aerobic endurance and muscle explosiveness: a synergistic approach
H Yun,Frontiers in nutrition,2024
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Toxicological and histopathological alterations in the heart of young and adult albino rats exposed to mosquito coil smoke.
AES Abdrabouh - 2023 - researchsquare.com,researchsquare,2023
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Efficacy of a Methanolic Extract of Adansonia digitata Leaf in Alleviating Hyperglycemia, Hyperlipidemia, and Oxidative Stress of Diabetic Rats
Hossam Ebaid, et al,Biomed research international,2019
靶點(diǎn)詳情
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功能：
Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
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基因功能參考文獻(xiàn)：
- Increases in plasma erythropoietin and erythropoietin receptor activation are mechanisms implicated in the increase of plasma FGF23 in acute kidney injury. PMID: 29395333
- Epo and Epo-Receptor are expressed in the spiral ganglion neurons (SGNs) of the inner ear of the rat, and Epo adenovirus vector (AdEpo) can decrease the spontaneous apoptosis of SGNs, which may provide a basis for the prevention or alleviation of sensorineural hearing loss. PMID: 29436578
- EPO administration increased TMPRSS6 content in the plasma membrane-enriched fraction of liver homogenate; in iron-pretreated rats, this increase was abolished. The results confirm that iron pretreatment prevents the EPO-induced decrease in liver Hamp expression. PMID: 28282554
- Protection of erythropoietin markedly attenuated pyroptosis and apoptosis of neurons exposed to sevoflurane via Erk1/2-Nrf2/Bach1 signal pathway. PMID: 28189515
- protective effects of EPO may be mediated by nitric oxide and the expression of different NOS enzymes, especially iNOS isoform PMID: 28087117
- EPO receptor ligand dimeric peptides AF41676 and AF43136 administered before reperfusion are able to reduce infarct size in a rat model of AMI. PMID: 27521836
- The present study aimed to determine the expression of erythropoietin (EPO) and the EPO receptor (EPOR) in spiral ganglion neurons (SGNs) in the inner ear of rats of various ages, and the associated neuronal apoptosis and hearing alterations. PMID: 27959434
- Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation. PMID: 26178913
- Taken together, our results reveal that EPO and cEPO mediate MEF2 target gene expression via the regulation of HDAC5 phosphorylation at Ser259/498, and suggest that HDAC5 could be a mechanism contributing to the therapeutic actions of EPO and cEPO. PMID: 26777998
- this study is the first to elucidate the detailed mechanism by which AhR plays an indispensable role in the suppression of HIF activation by IS. PMID: 26561638
- the hypercapnic hypoxia causes maximum activation of the synthesis and accumulation of erythropoietin HIF-1alpha, and permissive hypercapnia enhances their content more than hypoxic exposure. PMID: 26852593
- Results of the present study demonstrate the neuroprotective, anti-inflammatory and antioxidant effects of EPO in a rotenone-induced neurodegenerative animal model. PMID: 25464888
- Vitamin A deficiency leads to ineffective erythropoiesis by the down-regulation of renal erythropoietin expression in the kidney, resulting in erythrocyte malformation and the consequent accumulation of the heme group in the spleen. PMID: 24998947
- EPO partially rescued insulin-stimulated AKT activation, reduced oxidative stress, and restored heat-shock protein 72 expression in soleus muscles. PMID: 25767056
- Results showed that the inhibitory effects of TNFa on the hypoxia-induced upregulation of erythropoietin expression are mediated primarily by HIF-2a rather than HIF-1a PMID: 25283246
- Data suggest a potential positive effect of erythropoietin (EPO) on hepatocytes (HCs) proliferation during liver regeneration after a partial hepatectomy. PMID: 24928528
- HIF-1alpha/EPO hypoxic signal transduction may initiate hippocampal neurogenesis following hypoxia-ischemia PMID: 25935977
- The present study suggests that EPO can exert a cardioprotective effect on DOX-induced cardiotoxicity which may be associated with improving MMP-2/TIMP-2 imbalance. PMID: 24685074
- Epidermal growth factor and erythropoietin infusion accelerate functional recovery in combination with rehabilitation. PMID: 24763928
- In IN-IC cells, hypoxia increased mRNA expression of erythropoietin, erythropoietin concentration in the medium and protein expression of HIF PHD2. PMID: 24832733
- After 2-weeks of hypoxia, erythropoietin transcripts and erythropoietin plasma levels were significantly increased in hypoxic animals compared with control animals. PMID: 24524370
- rMSCs transplantation could be a new therapy for degenerative retinal diseases since it can protect and rescue RPE and retinal neurons, while EPO gene modification to rMSCs could be an even better option. PMID: 23971737
- Erythropoietin prevents lymphoid apoptosis but has no effect on survival in experimental sepsis. PMID: 23728385
- EPO induces the expression of myelin genes in oligodendrocytes and this effect requires the presence of EPOR. PMID: 23821361
- EPO significantly suppresses the enhanced apoptosis and activity of caspase 3 that are induced by 6-hydroxydopamine. PMID: 22294054
- 11 weeks of endurance training increased erythrocyte deformability. Epo and EpoR may contribute to the decreased morphological index and deformability indicator in erythrocytes during endurance training in rats. PMID: 22495316
- EPO potently protects against cardiopulmonary bypass-induced acute renal injury by inhibiting expression of NF-kappaB p65 and inflammatory response. PMID: 22659586
- EPO induces a significant decrement in the ohmic airway resistance. PMID: 22766248
- Pyruvate activation of the hypoxia-inducible factor-1alpha-EPO signaling cascade in neurons and glia could protect the brain from ischemia-reperfusion injury PMID: 22282883
- Ligustilide exerts neuroprotective effects against I/R injury by promoting EPO transcription via an ERK signalling pathway and inhibiting RTP801 expression. PMID: 21410687
- A protective cascade for erythropoietin (EPO) relies upon modulation of Wnt1, Akt1, FoxO3a, GSK-3beta, beta-catenin, and mitochondrial apoptotic pathways for the development of new strategies against DM vascular complications. PMID: 21443457
- Our work identifies novel pathways for EPO in the vascular system that can govern the activity of SIRT1 PMID: 21722091
- EPO, hCG, or their combination ameliorate cardiac remodeling post-myocardial infarct in a rat model PMID: 21707946
- Epo combined with granulocyte colonystimulating factor enhanced Mmp2 expression in mesenchymal stem cells, promoted motility and activated the ERK1/2 signaling pathway. PMID: 21461559
- EPO mediates brain-derived neurotrophic factor-dependent sonic hedgehog (SHH) expression and 3-nitropropionic acid (3-NP) resistance because suppression of EPO activity by soluble EPO-receptor abolishes BDNF-mediated SHH induction and 3-NP resistance. PMID: 20580927
- The expression of erythropoietin in the hippocampus increased from 3 to 18 months and decreased from 18 to 30 months. PMID: 19779256
- EPO may play a role in normal spinal cord development by regulating cell proliferation and apoptosis. PMID: 20167254
- Changes in EPO concentration [EPO] was evaluated every hour after single exposure of 10% O(2) for 120 min in order to determine a peak level of [EPO]. PMID: 20204781
- Intermittent hypoxic exposure can enhance serum hypoxia inducible factor-1 alpha and erythropointin levels. PMID: 20104680
- Our results support a model in which Epo promotes Schwann cell migration and assembly of the provisional extracellular matrix in the injured peripheral nerve by its effects on integrin recruitment to the cell surface and local FN production. PMID: 19705458
- All regions of kidney express erythropoietin receptors. Review. PMID: 12381912
- role in preventing the induction of Apaf-1 and preserving mitochondrial membrane potential in conjunction with enhanced Bcl-XL expression. EPO also inhibits the activation of caspase-9. PMID: 12621307
- EPO inhibits apoptosis in ventricular myocytes exposed to hypoxia, through an Akt-dependent pathway PMID: 12927817
- Erythropoietin may provide benefit after hypoxic-ischemic events in the developing brain, a major contributor to static encephalopathy and cerebral palsy. PMID: 13129593
- Hypoxic acclimatization can inhibit the increment of erythropoietin gene expression induced by acute hypoxia in rat hepatic and renal tissues. PMID: 14706194
- EPO mediates the classic neurotrophic effects of proliferation, differentiation and maintenance in a dopaminergic cell line. PMID: 15050726
- Treatment of neural progenitor cells with erythropoietin stimulated progenitor cell differentiation into neurons, but not astrocytes. PMID: 15167538
- These data demonstrate that EPO can promote differentiation of neuronal stem cells into astrocytes in an EPO receptor dependent manner, and this effect may be associated with the activation of ERK kinase and NF-kappaB pathway. PMID: 15249201
- Age-related decreases in Erythropoietin may contribute to degenerative events following age-related decreases in brain flow and oxygen supply. PMID: 15262216
- Study demonstrates how in response to axonal injury, rat periaxonal Schwann cells release erythropoietin (EPO), which via EPO receptor binding on neurons, prevents axonal degeneration. PMID: 15470751
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亞細(xì)胞定位：
Secreted.
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蛋白家族：
EPO/TPO family
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組織特異性：
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
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數(shù)據(jù)庫鏈接：
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