Rat Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit

說明書下載

中文名稱：

大鼠上皮中性粒細胞活化肽78(ENA-78/CXCL5)酶聯免疫試劑盒
貨號：

CSB-E08179r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

大鼠上皮中性粒細胞活化肽78(ENA-78/CXCL5)酶聯免疫試劑盒（CSB-E08179r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CXCL5含量。CXCL5 屬于趨化因子，在炎癥反應、腫瘤發展等過程中有重要作用。其研究機制主要涉及與受體結合，激活下游信號通路，促進細胞遷移、增殖等。在腫瘤領域，它可影響腫瘤微環境，促進血管生成，為相關疾病治療提供潛在靶點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，靈敏度為0.078 ng/mL。本試劑盒適用于科研領域中對CXCL5在炎癥模型、免疫調節或疾病機制研究中的動態變化分析，如通過血清或血漿樣本評估系統性炎癥狀態，或利用組織勻漿探究局部CXCL5表達特征，為探索相關信號通路、藥物干預效果等提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Cxcl5 ELISA Kit; Scyb5C-X-C motif chemokine 5 ELISA Kit; Cytokine LIX ELISA Kit; Small-inducible cytokine B5 ELISA Kit
縮寫：

CXCL5
Uniprot No.：

P97885
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	2.442	2.474	2.529	2.446	2.487	2.542
SD	0.035	0.033	0.038	0.041	0.051	0.048
CV(%)	5.486	5.116	5.784	6.416	7.870	7.273

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

83-91

1:2

Average %

104

Range %

98-108

1:4

Average %

Range %

87-96

1:8

Average %

Range %

91-99

回收率：

The recovery of rat ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	94	87-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



ng/ml	OD1	OD2	Average	Corrected
20	2.746	2.689	2.718	2.607
10	2.000	1.899	1.950	1.839
5	1.190	1.171	1.181	1.070
2.5	0.651	0.640	0.646	0.535
1.25	0.366	0.354	0.360	0.249
0.625	0.263	0.257	0.260	0.149
0.312	0.172	0.178	0.175	0.064	0.064
0	0.110	0.112	0.111

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

產品評價

靶點詳情

最新研究進展：

CXCL5，也稱為ENA-78，是一種重要的趨化因子蛋白，可誘導中性粒細胞和其他白細胞向感染或炎癥部位遷移。最近的研究表明，CXCL5在多種腫瘤中的表達與惡性程度和預后密切相關，并且可作為潛在的治療靶點。
功能：

May participate in the recruitment of inflammatory cells by injured or infected tissue.
基因功能參考文獻：
1. CRH suppressed CXCL5 production in glia via CRHR1 in the hippocampus of male rats. PMID: 29126185
2. up-regulation of spinal CXCL5 and CXCR2 is involved in neuropathic pain after nerve injury, through regulating GSK-3beta activity in rats. PMID: 27717828
3. Analysis of mitogen-activated protein kinases (MAPK) and glycogen synthase kinase (GSK3) pathways showed that they are involved in mechanisms of neuronal apoptosis in response to the depletion of CXCL5/LIX signaling. PMID: 22034072
4. Data demonstrate that the chemokine CXCL5 is a peripheral mediator of UVB-induced inflammatory pain, likely in humans as well as rats. PMID: 21734176
5. Disrupted expression of CXCL5 in colorectal cancer is associated with rapid tumor formation in rats and poor prognosis in patients. PMID: 18413816
顯示更多

收起更多
亞細胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
數據庫鏈接：

KEGG: rno:60665

STRING: 10116.ENSRNOP00000003823

UniGene: Rn.44449

Rat Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit

產品詳情

產品評價

相關產品

靶點詳情

Most popular with customers

產品中心

信號通路

技術信息

聯系信息