Rat Cystatin C,Cys-C ELISA Kit

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中文名稱：

大鼠半胱氨酸蛋白酶抑制劑/胱抑素C(Cys-C)酶聯免疫試劑盒
貨號：

CSB-E08385r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠半胱氨酸蛋白酶抑制劑/胱抑素C(Cys-C)酶聯免疫試劑盒（CSB-E08385r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、勻漿樣本中的CST3含量。CST3 即半胱氨酸蛋白酶抑制劑 C，是一種內源性半胱氨酸蛋白酶抑制劑。在人體生理中起穩定蛋白酶平衡等作用。研究發現其與神經系統疾病、腎臟疾病等密切相關，如可作為評估腎小球濾過功能指標，機制可能與調節體內蛋白酶活性和炎癥反應等有關。試劑盒檢測范圍為0.312 ng/ml-20 ng/ml，靈敏度為0.078 ng/ml。適用于科研領域中對腎臟疾病模型、藥物代謝研究或組織損傷相關實驗中Cys-C表達水平的動態監測，幫助研究者分析其與腎功能狀態、炎癥進程或細胞凋亡的關聯性。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Cst3 ELISA Kit; Cystatin-C ELISA Kit; Cystatin-3 ELISA Kit
縮寫：

CST3
Uniprot No.：

P14841
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, homogenates
檢測范圍：

0.312 ng/ml-20 ng/ml
靈敏度：

0.078 ng/ml
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	2.490	2.550	2.588	2.467	2.527	2.555
SD	0.031	0.039	0.046	0.041	0.048	0.056
CV(%)	3.407	4.225	4.941	4.530	5.229	6.061

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:2

Average %

Range %

93-99

1:4

Average %

Range %

90-96

1:8

Average %

Range %

91-104

1:16

Average %

Range %

89-95

回收率：

The recovery of rat Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
20	2.564	2.587	2.576	2.404
10	1.923	1.934	1.929	1.757
5	1.329	1.368	1.349	1.177
2.5	0.912	0.937	0.925	0.753
1.25	0.582	0.598	0.590	0.418
0.625	0.412	0.436	0.424	0.252
0.312	0.311	0.317	0.349	0.177
0	0.170	0.174	0.172

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Abatement by Alhagi maurorum of lead-induced nephrotoxicity in rats: emphasis on Nrf2/HO-1 MM Algandaby,Future Journal of Pharmaceutical Sciences,2025
Sanqi oral solution ameliorates renal fibrosis by suppressing fibroblast activation via HIF-1α/PKM2/glycolysis pathway in chronic kidney disease D Hu, L Wang, Y Zhang, X Liu, Z Lu, H Li,Journal of Ethnopharmacology,2024
Linagliptin mitigates cisplatin-induced kidney impairment via mitophagy regulation in rats, with emphasis on SIRT-3/PGC-1α, PINK-1 and Parkin-2 MGED Ewees, G Mostafa-Hadeab, S Saber,Toxicology and Applied Pharmacology,2024
Clopidogrel protects against gentamicin-induced nephrotoxicity through targeting oxidative stress, apoptosis, and coagulation pathways AA Akila, RA Gad, MGED Ewees,Naunyn-Schmiedeberg's Archives of Pharmacology,2024
Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats MGED Ewees,Environmental toxicology and pharmacology,2023
Rosuvastatin and diosmetin inhibited the HSP70/TLR4 /NF-κB p65/NLRP3 signaling pathways and switched macrophage to M2 phenotype in a rat model of acute kidney injury induced by cisplatin F Feng,Biomedicine & pharmacotherapy,2024
Anandamide modulates WNT-5A/BCL-2, IP3/NFATc1, and HMGB1/NF-κB trajectories to protect against mercuric chloride-induced acute kidney injury DM Abdallah,Scientific reports,2023
Effect of perinatal exposure to glyphosate and its mixture with 2, 4-D and dicamba on rat dam kidney and thyroid function and offspring's health AO Docea,Environmental research,2023
2-Methoxyestradiol TPGS Micelles Attenuate Cyclosporine A-Induced Nephrotoxicity in Rats through Inhibition of TGF-β1 and p-ERK1/2 Axis MW Al-Rabia,Antioxidants,2022
Omeprazole Prevents Colistin-Induced Nephrotoxicity in Rats: Emphasis on Oxidative Stress, Inflammation, Apoptosis and Colistin Accumulation in Kidneys MZ Nasrullah,Pharmaceuticals,2022
Renoprotective Effect of Vardenafil and Avanafil in Contrast-Induced Nephropathy: Emerging Evidence from an Animal Model IE Zisis,Journal of Personalized Medicine,2022
Exogenous H2S Ameliorates High Salt-Induced Hypertension by Alleviating Oxidative Stress and Inflammation in the Paraventricular Nucleus in Dahl S Rats Yingying Liao,Cardiovascular Toxicology,2022
Involvement of NOX-4/JAK/STAT pathway in the protective effect of aprepitant against diclofenac-induced renal toxicity HM Hafez,Life Sciences,2022
Renoprotective effects of tropisetron through regulation of the TGF-β1, p53 and matrix metalloproteinases in streptozotocin-induced diabetic rats B Pourheydar,Chemico-Biological Interactions,2021
The renoprotective effect of glycyrrhizic acid in insulin-resistant rats exposed to aluminum involves the inhibition of TLR4/NF-κB signaling pathway NA Emara,Naunyn-Schmiedeberg's Archives of Pharmacology,2020
Protective Effects of Combination of Radix Astragali and Radix Salviae Miltiorrhizae on Kidney of Spontaneously Hypertensive Rats and Renal Intrinsic Cells Li W, et al,Chinese Journal of Integrative Medicine,2019
Renoprotective effect of Zhenwu decoction against renal fibrosis by regulation of oxidative damage and energy metabolism disorder Li S.et al,Scientific Reports,2018
Tempol, a superoxide dismutase mimetic agent, reduces cisplatin-induced nephrotoxicity in rats Mohamed G. Ewees.et al,drug and chemical toxicology,2018
Ameliorative effect of ursolic acid on renal fibrosis in adenine-induced chronic kidney disease in rats RichaThakur.et al,Biomedicine & Pharmacotherapy,2018
Stimulation of ACE2/ANG/Mas Axis by Diminazene Ameliorates Alzheimer's Disease in the D-Galactose-Ovariectomized Rat Model: Role of PI3K/Akt Kamel AS.et al,Mol Neurobiol,2018
Nandrolone administration with or without strenuous exercise promotes overexpression of nephrin and podocin genes and induces structural and functional Tofighi A.et al,Toxicol Lett,2017
Relevance of cystatin-C, N-acetylglucosaminidase, and Interleukin-18 with the diagnosis of acute kidney injury induced by cadmium in rats Mohamed Ali OS.et al,J Biochem Mol Toxicol.,2017
Transplantation of insulin-producing cells to treat diabetic rats after 90% pancreatectomy Ya-Bin Yu. et al,World J Gastroenterol.,2015

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最新研究進展：

CST3是一種由人體細胞產生的小分子蛋白質，也被稱為半胱氨酸蛋白酶抑制劑或淀粉樣蛋白沉積物抑制劑。它在細胞凋亡、免疫調節、組織修復和生殖系統發育等方面發揮重要作用。最近的研究表明，CST3與多種疾病有關，包括神經退行性疾病、腎臟疾病和腫瘤。
功能：

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.
基因功能參考文獻：
1. Tanshinone IIA protects against cardiac hypertrophy in spontaneously hypertensive rats by inhibiting the Cys-C/Wnt signaling pathway. PMID: 28053285
2. These results indicate that Cd increases the urinary excretion of cystatin C, and they suggest that this effect may involve disruption of megalin-mediated uptake of cystatin C by epithelial cells of the proximal tubule. PMID: 26715107
3. High cystatin C is associated with proteinuria. PMID: 21551085
4. Triiodothyronine-induced increase in the production of cystatin C may be related to an increased cell metabolism and proteolysis control demand. PMID: 22360852
5. CysC plays a protective role under conditions of neuronal challenge by inducing autophagy via mTOR inhibition PMID: 20352108
6. Oxidants induce elevated cystatin C production from cardiac myocytes. Cystatin C plays a role in cardiac extracellular matrix remodelling. PMID: 20489058
7. We found several novel glycoforms of cystatin c in rat hippocampal stem cells, suggesting that N- and O-linked glycans with sialic acid are attached to cystatin C. PMID: 15197734
8. These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death. PMID: 16140167
9. endometrial expression of the cysteine protease inhibitor, Cst3, is modulated by estrogen during the peri-implantation period PMID: 16935259
10. These studies suggest alternate pathways and potential therapeutic targets of cell death associated with oxidative stress, CC, and lysosomal dysregulation. PMID: 17241700
11. Finally, in vivo studies using kidney-specific megalin knockout mice provide evidence that megalin mediates proximal tubular uptake of cystatin C. We conclude that megalin is an endocytic receptor of cystatin C in PTC. PMID: 17462596
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亞細胞定位：

Secreted.
蛋白家族：

Cystatin family
數據庫鏈接：

KEGG: rno:25307

STRING: 10116.ENSRNOP00000007175

UniGene: Rn.106351

Rat Cystatin C,Cys-C ELISA Kit

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