Rat Cluster of Differentiation 68, CD68 ELISA kit

Rat Cluster of Differentiation 68, CD68 ELISA kit

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中文名稱：

大鼠CD68分子，CD68酶聯(lián)免疫試劑盒
貨號：

CSB-E13297r
規(guī)格：

96T/48T
價(jià)格：

￥3900/￥2500
促銷：
其他：

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大鼠CD68分子，CD68酶聯(lián)免疫試劑盒（CSB-E13297r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD68含量。CD68是一種在單核細(xì)胞、巨噬細(xì)胞等免疫細(xì)胞上高度表達(dá)的跨膜糖蛋白。它參與細(xì)胞內(nèi)吞、信號傳導(dǎo)等多種生理過程。當(dāng)前研究聚焦其在炎癥、腫瘤等疾病中的作用機(jī)制，有望為相關(guān)疾病的診斷和治療提供新靶點(diǎn)和思路。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于多種科研場景，包括巨噬細(xì)胞活化狀態(tài)分析、炎癥性疾病動物模型研究（如關(guān)節(jié)炎或動脈粥樣硬化）、組織修復(fù)過程中巨噬細(xì)胞浸潤評估等實(shí)驗(yàn)需求。支持科研人員高效開展免疫細(xì)胞功能研究、病理機(jī)制探索及藥物干預(yù)效果評價(jià)等實(shí)驗(yàn)，尤其適用于心血管、腫瘤微環(huán)境及免疫代謝相關(guān)領(lǐng)域的樣本檢測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CD68
Uniprot No.：

Q4FZY1
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD68 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-96
1:2	Average %	102
	Range %	98-105
1:4	Average %	91
	Range %	87-95
1:8	Average %	95
	Range %	91-98

回收率：

The recovery of rat CD68 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	101	94-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.210	2.188	2.199	2.063
20	1.484	1.444	1.464	1.328
10	0.971	1.008	0.990	0.854
5	0.622	0.645	0.634	0.498
2.5	0.378	0.387	0.383	0.247
1.25	0.304	0.299	0.302	0.166
0.625	0.222	0.212	0.217	0.081
0	0.138	0.134	0.136

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Anthocyanins Protect Hepatocytes against CCl4-Induced Acute Liver Injury in Rats by Inhibiting Pro-inflammatory mediators, Polyamine Catabolism, Lipocalin-2, and Excessive Proliferation of Kupffer Cells Popovic D, et al,Antioxidants,2019
Hypothermic oxygenated perfusion (HOPE) protects from biliary injury in a rodent model of DCD liver transplantation Andrea Schlegel et al,Journal of Hepatology,2013

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.