Rat Chordin-like protein 1(CHRDL1) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠腱蛋白樣蛋白1(CHRDL1)酶聯(lián)免疫試劑盒（CSB-EL005378RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CHRDL1含量。試劑盒檢測范圍為0.45 ng/mL-30 ng/mL，適用于基礎(chǔ)科研中 CHRDL1 表達調(diào)控機制研究、骨骼發(fā)育模型建立、神經(jīng)再生實驗中的蛋白動態(tài)分析，或病理狀態(tài)下組織修復(fù)相關(guān)研究。96 孔板規(guī)格設(shè)計滿足高通量篩選需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Chrdl1 ELISA Kit; Kjr ELISA Kit; Ng1Chordin-like protein 1 ELISA Kit; Kohjirin ELISA Kit; Neuralin-1 ELISA Kit; Neurogenesin-1 ELISA Kit; Ventroptin ELISA Kit
縮寫：

CHRDL1
Uniprot No.：

Q76LD0
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.45 ng/mL-30 ng/mL
靈敏度：

0.11 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-96
1:2	Average %	96
	Range %	91-104
1:4	Average %	97
	Range %	92-105
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of rat CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-108
EDTA plasma (n=4)	91	86-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. Plays a role during anterior segment eye development.
基因功能參考文獻：
1. Ng1 gene could promote the proliferation of endogenous neural stem cells and protect injured neurons following spinal cord injury. PMID: 20459000
2. Neurogenesin-1 contributes to neuronal differentiation of neural stem cells in the adult brain PMID: 14684875
亞細胞定位：

Secreted.
數(shù)據(jù)庫鏈接：

KEGG: rno:363455

STRING: 10116.ENSRNOP00000006008

UniGene: Rn.211154