Rat Cathepsin S(CTSS) ELISA kit

Rat Cathepsin S(CTSS) ELISA kit

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中文名稱：

大鼠組織蛋白酶S(CTSS)酶聯免疫試劑盒
貨號：

CSB-EL006204RA
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠組織蛋白酶S(CTSS)酶聯免疫試劑盒（CSB-EL006204RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CTSS含量。CTSS（組織蛋白酶S）是一種溶酶體半胱氨酸蛋白酶，參與抗原呈遞和細胞外基質重塑。其在多種疾病如皮膚纖維瘤、肝纖維化中發揮作用，成為潛在的治療靶點。研究顯示，CTSS通過調節細胞外基質成分，影響疾病進程。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于體外實驗中的蛋白表達水平監測、疾病機制研究及藥物干預效果評價等科研領域，滿足細胞實驗、動物模型組織分析等多種研究需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ctss ELISA Kit; Cathepsin S ELISA Kit; EC 3.4.22.27 ELISA Kit
縮寫：

CTSS
Uniprot No.：

Q02765
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CTSS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-98
1:2	Average %	97
	Range %	93-105
1:4	Average %	90
	Range %	85-94
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of rat CTSS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	98-110
EDTA plasma (n=4)	84	80-89

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.563	2.457	2.510	2.395
500	1.997	2.076	2.037	1.922
250	1.378	1.404	1.391	1.276
125	0.877	0.821	0.849	0.734
62.5	0.567	0.554	0.561	0.446
31.25	0.358	0.331	0.345	0.230
15.6	0.227	0.240	0.234	0.119
0	0.113	0.117	0.115

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

P2X7 receptor mediated release of microglial prostanoids and miRNAs correlates with reversal of neuropathic hypersensitivity in rats R Staal,European journal of pain,2023
Inhibition of P2X7 receptors by Lu AF27139 diminishes colonic hypersensitivity and CNS prostanoid levels in a rat model of visceral pain R Staal,/,2022

功能：

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
基因功能參考文獻：
1. Findings demonstrate that neuronal reactive oxygen species promote maturation of microglial CatS which facilitates activation of NMDA in the spinal dorsal horn. PMID: 28039043
2. Inhibition of cathepsin S release is one mechanism that is responsible for purinergic P2X7 antagonist efficacy in neuropathic pain. PMID: 20629190
3. Activated rat hepatic stellate cells express the class II transactivator, the invariant chain (CD74), the MHC class II molecules, as well as cathepsin S, all of which are known to be responsible for the initial steps of successful antigen presentation. PMID: 17178165
4. endogenous CatS released by peripheral macrophages contributes to the maintenance of neuropathic hyperalgesia following nerve injury PMID: 17250968
5. There is an altered expression of cathepsin S and cathepsin X in the spinal cord of a neuropathic pain model; including cellular distribution and pain development. PMID: 18700000
6. under neuropathic conditions, noxious stimulation of primary afferent fibers induces release of CatS from microglia, which liberates FKN from dorsal horn neurons, thereby contributing to the amplification and maintenance of chronic pain PMID: 19474321
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亞細胞定位：

Lysosome. Secreted. Cytoplasmic vesicle, phagosome.
蛋白家族：

Peptidase C1 family
組織特異性：

Highest levels occur in the ileum followed by spleen, brain, thyroid, ovary and uterus. Low levels are found in the liver, kidney, jejunum and lung with lowest levels in the heart.
數據庫鏈接：

KEGG: rno:50654

UniGene: Rn.11347

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.