Rat Cathepsin K,cath-K ELISA Kit
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中文名稱：大鼠組織蛋白酶K(cath-K)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E09439r
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規(guī)格：96T/48T
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價(jià)格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠組織蛋白酶K(cath-K)酶聯(lián)免疫試劑盒（CSB-E09439r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CTSK含量。CTSK（組織蛋白酶K）是一種在骨重塑過(guò)程中起關(guān)鍵作用的酶，主要在破骨細(xì)胞中表達(dá)。其研究機(jī)制涉及降解骨細(xì)胞外基質(zhì)，促進(jìn)骨吸收。CTSK在多種癌癥中表達(dá)，可能促進(jìn)腫瘤細(xì)胞增殖、侵襲和遷移。研究CTSK有助于開發(fā)新的治療策略。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于大鼠骨代謝研究、骨骼疾病動(dòng)物模型評(píng)估及骨靶向藥物作用機(jī)制探索等科研領(lǐng)域，可為骨生物學(xué)、病理機(jī)制研究及藥物開發(fā)提供穩(wěn)定可靠的定量分析支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
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別名：Ctsk ELISA Kit; Cathepsin K ELISA Kit; EC 3.4.22.38 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：15.6 pg/mL-1000 pg/mL
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靈敏度：3.9 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 104 Range % 100-107 1:2 Average % 99 Range % 95-103 1:4 Average % 85 Range % 80-90 1:8 Average % 107 Range % 103-110 -
回收率：
The recovery of rat cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 86 83-89 EDTA plasma (n=4) 95 90-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1000 2.801 2.765 2.783 2.682 500 2.342 2.204 2.273 2.172 250 1.343 1.406 1.375 1.274 125 0.679 0.701 0.690 0.589 62.5 0.399 0.405 0.402 0.301 31.2 0.251 0.242 0.247 0.146 15.6 0.166 0.164 0.165 0.064 0 0.102 0.100 0.101 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β‐catenin signaling pathway in ovariectomized rats H Liu,Phytother Res,2020
- Early changes in serum osteocalcin and body weight are predictive of implant fixation in a rat model of implant loosening Wilson B M, et al,Journal of Orthopaedic Research,2019
- Research article Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and trabecular bone loss Azza M. El-Wakf,Journal of Innovations in Pharmaceutical and Biological Sciences,2019
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靶點(diǎn)詳情
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功能：Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen.
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基因功能參考文獻(xiàn)：
- the present study identified icariin as a novel therapeutic agent in DXM-induced cartilage injury in rats, and revealed that the activation of miR-206 targeting of cathepsin K may be responsible for the chondroprotective efficacy of icariin PMID: 29207029
- cathepsin K, in both its secreted and lysosomal forms, may play a role in the degradation of Sertoli cell residual bodies PMID: 14585819
- In conclusion, according to its ability to modulate hypotensive effects of kinins, we propose that cathepsin K is a kininase of biological relevance. PMID: 17181996
- Comparison of the S2 site between rat and human cathepsin K sequences indicated that two S2 residues at Ser134 and Val160 in rat are varied to Ala and Leu, respectively, in the human enzyme. PMID: 18664521
- The expression pattern of Ctsk in osteoclasts in physiological root resorption in the rat molar was determined. PMID: 18974601
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亞細(xì)胞定位：Lysosome. Secreted. Apical cell membrane; Peripheral membrane protein; Extracellular side.
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蛋白家族：Peptidase C1 family
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數(shù)據(jù)庫(kù)鏈接：
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