Rat Caspase-9,Casp-9 ELISA Kit
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中文名稱：大鼠胱天蛋白酶9(Casp-9)酶聯(lián)免疫試劑盒
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貨號：CSB-E08863r
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規(guī)格：96T/48T
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價格：￥3900/￥2500
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促銷：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠胱天蛋白酶9(Casp-9)酶聯(lián)免疫試劑盒（CSB-E08863r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Casp-9含量。Casp-9即半胱天冬酶9，在細(xì)胞凋亡機(jī)制中有重要背景。它是凋亡途徑關(guān)鍵成員，作為凋亡啟動者，被細(xì)胞色素C激活后，能招募相關(guān)蛋白形成復(fù)合物，激活下游Caspases級聯(lián)反應(yīng)，引發(fā)細(xì)胞凋亡，是腫瘤等疾病研究的潛在靶點(diǎn)。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，可廣泛應(yīng)用于基礎(chǔ)科研領(lǐng)域，如凋亡信號通路機(jī)制研究、藥物開發(fā)中的靶點(diǎn)驗(yàn)證或疾病模型中Caspase - 9活性變化的動態(tài)監(jiān)測，為體外實(shí)驗(yàn)提供穩(wěn)定可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：Casp-9
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：62.5 pg/mL-4000 pg/mL
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靈敏度：15.6 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Cell Biology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 90-99 1:2 Average % 95 Range % 91-102 1:4 Average % 98 Range % 94-103 1:8 Average % 102 Range % 96-106 -
回收率：
The recovery of rat Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-98 EDTA plasma (n=4) 100 96-104 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 4000 1.846 1.946 1.896 1.752 2000 1.442 1.542 1.492 1.348 1000 0.986 1.016 1.001 0.857 500 0.546 0.556 0.551 0.407 250 0.320 0.330 0.325 0.181 125 0.266 0.278 0.272 0.128 62.5 0.204 0.207 0.206 0.062 0 0.143 0.144 0.144 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Lagerstroemia indica methanolic extract alleviates CCl4-induced liver toxicity via modulation of Nrf2 signaling pathway in rats J Nisar, M Sarwar, SAR Shah, S Yasmin,Asian Pacific Journal of Tropical Biomedicine,2025
- AA Saati,Brain Sciences,2025
- Protective Effect of Whey Protein Supplement Against Rotenone Induced Motor Dysfunction in a Rat Model of Parkinson Disease P Zarei, B Amirpour-Najafabadi,Advanced Biomedical Research,2024
- Echis ocellatus venom-induced sperm functional deficits, pro-apoptotic and inflammatory activities in male reproductive organs in rats: antagonistic role of kaempferol BS Ajisebiola, AA Toromade, JO Oladele,BMC Pharmacology and Toxicology,2024
- Rhoifolin protects cisplatin mediated pulmonary toxicity via attenuation of oxidative stress, inflammatory response, apoptosis and histopathological damages A Akbar,JOURNAL OF KING SAUD UNIVERSITY SCIENCE,2024
- Cardio-nephrotoxicity mediated by Echis ocellatus venom and its amelioration through kaempferol’s suppressive effect on oxidative stress, inflammation, and apoptosis expression BS Ajisebiola,Clinical Phytoscience,2024
- Diosmin protects the testicles from doxorubicin-induced damage by increasing steroidogenesis and suppressing oxido-inflammation and apoptotic mediators MO Oyovwi,International journal of biochemistry and molecular biology,2024
- Evaluation of Possible Ameliorative Role of Robinetin to Counteract Polystyrene Microplastics Instigated Renal Toxicity in Rats MU Ijaz,/,2024
- Ameliorative Effect of Thymoquinone and Thymoquinone Nanoparticles against Diazinon-Induced Hepatic Injury in Rats: A Possible Protection Mechanism WM Nassar,Toxics,2023
- Exposure to bromoxynil octanoate herbicide induces oxidative stress, inflammation, and apoptosis in testicular tissue via modulating NF-кB pathway MMF El-Nagar,Food and chemical toxicology,2023
- Parthenolide repressed endometriosis induced surgically in rats: Role of PTEN/PI3Kinase/AKT/GSK-3β/β-catenin signaling in inhibition of epithelial mesenchymal transition SL Kabil,Life sciences,2023
- Ameliorative potential of sinensetin against paraquat induced renal damage by regulating oxidative inflammatory apoptotic and histopathological profile in male albino rats J Zhao,/,2024
- Simvastatin ameliorates testosterone-induced prostatic hyperplasia in rats via modulating IGF-1/PI3K/AKT/FOXO signaling NH El-Shafei,European journal of pharmacology,2023
- Therapeutic Efficacy of Chrysoeriol on Doxorubicin-induced Liver Damage by Improving Biochemical and Histological Profile in Rats MU Ijaz,SciEP,2023
- Evaluation of the Possible Protective Role of Nobiletin against Arsenic-Induced Liver Damage in Male Albino Rats MU Ijaz,Toxics,2023
- Diosmetin alleviates nonylphenol-induced liver damage by improving biochemical, inflammatory, apoptotic and histological profile in rats R Azmat,Journal of King Saud University - Science,2022
- Fisetin Attenuates Arsenic-Induced Hepatic Damage by Improving Biochemical, Inflammatory, Apoptotic, and Histological Profile: In Vivo and In Silico Approach M Umar,Evidence-Based Complementary and Alternative Medicine,2022
- Methanol Extract of Chasmanthera dependens Stem Mitigates against Mechanisms Involved in Piroxicam-induced Liver Damage in Rat TS Abiola,Journal of Complementary and Alternative Medical Research,2020
- Formononetin Upregulates Nrf2/HO-1 Signaling and Prevents Oxidative Stress, Inflammation, and Kidney Injury in Methotrexate-Induced Rats Aladaileh S H, et al,Antioxidants,2019
- Bacoside a Attenuates Nephrotoxicity and Acute Kidney Injury in Male Albino Rats Induced by Cisplatin Gong Xiao, et al,International Journal of Pharmacology,2019
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