Rat Cardiac Troponin T(cTn-T)ELISA kit

Rat Cardiac Troponin T(cTn-T)ELISA kit

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文獻引用 (28) 說明書下載

中文名稱：

大鼠心肌肌鈣蛋白T(cTn-T)酶聯免疫試劑盒
貨號：

CSB-E16443r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠心肌肌鈣蛋白T(cTn-T)酶聯免疫試劑盒（CSB-E16443r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的cTn-T含量。cTn-T即心肌肌鈣蛋白T，是心肌損傷的特異性標志物。其背景在于臨床上用于診斷心肌梗死等疾病。研究機制方面，心肌受損時，cTn-T釋放入血，通過檢測血液中其含量變化，可反映心肌損傷程度、范圍及評估預后等。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，可廣泛應用于心臟疾病動物模型研究，如心肌缺血再灌注損傷、藥物心臟毒性評估、心肌炎或心力衰竭機制探索等科研領域，為體外定量分析心肌損傷提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Tnnt2 ELISA Kit; Troponin T ELISA Kit; cardiac muscle ELISA Kit; TnTc ELISA Kit; Cardiac muscle troponin T ELISA Kit; cTnT ELISA Kit
縮寫：

cTn-T
Uniprot No.：

P50753
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-T in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-97
1:2	Average %	87
	Range %	81-94
1:4	Average %	93
	Range %	87-100
1:8	Average %	95
	Range %	89-102

回收率：

The recovery of rat cTn-T spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-105
EDTA plasma (n=4)	95	88-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.187	2.102	2.145	1.986
400	1.731	1.641	1.686	1.527
200	1.254	1.204	1.229	1.070
100	0.926	0.879	0.903	0.744
50	0.593	0.554	0.574	0.415
25	0.302	0.279	0.291	0.132
12.5	0.241	0.227	0.234	0.075
0	0.165	0.153	0.159

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
基因功能參考文獻：
1. Replacement of the functionally corresponding N-terminal end portion of rat fast skeletal cardiac muscle (RfsTnT) into cardiac muscle troponin T (RcTnT), the observed functional differences associate with a sequence variation. PMID: 23357173
2. L48Q cTnC reduced crossbridge dependence of thin filament activation in cardiac muscle and changes in Ca(2+) sensitivity of force in response to changes in sarcomere length are at least partially dependent on properties of thin filament troponin. PMID: 22865385
3. cTnC, cTnI, cTnT and cTm are not only present in myofilaments of ventricular cardiomyocytes in culture but are also within their nuclei; significantly, these four proteins appear between days 3 and 5 in both myofilaments and nuclei PMID: 22364878
4. Early single cTnT measurement correlates with infarct size in rats, and a cutoff value of 5.1 mug/l provides good sensitivity and specificity to predict congestive heart failure with secondary pulmonary hypertension. PMID: 20537565
5. The time course of cTnT elevation was temporally associated with evidence of increased lipid peroxidation in the rat heart. PMID: 20711602
6. a novel role for cTnT as a dual-specificity sarcomeric A-kinase anchoring protein PMID: 21056973
7. Studies show that the rat Tnnt2-rtTA;TetO-Cre mice transgenic line a valuable genetic tool for analysis of spatiotemporal gene function and cardiomyocyte lineage tracing during developmental and postnatal period. PMID: 20014345
8. The mu-calpain-mediated proteolytic modification of TnT may act as an acute mechanism to adjust muscle contractility under stress conditions. PMID: 16981728
9. Overexpression of heat shock protein 27 protects against ischaemia/reperfusion-induced cardiac dysfunction via stabilization of troponin I and T. PMID: 18397962
10. Mdetabolic inhibition of cardiomyocytes induces a parallel release of intact Tnnt3 and its degradation products, starting only after onset of irreversible cardiomyocyte damage. PMID: 18721805
11. Study demonstrate profound energetic alterations in DEL-TNT hearts, supporting the notion that inefficient cellular ATP utilization contributes to the pathogenesis of HCM. PMID: 19189074
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蛋白家族：

Troponin T family
數據庫鏈接：

KEGG: rno:24837

STRING: 10116.ENSRNOP00000049297

UniGene: Rn.9965

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.