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Rat C-X-C motif chemokine 9(CXCL9) ELISA kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

大鼠趨化因子9(CXCL9)酶聯(lián)免疫試劑盒（CSB-EL006252RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CXCL9含量。CXCL9是重要的靶點。它屬趨化因子家族，在免疫調(diào)節(jié)中起關(guān)鍵作用。其研究機制主要聚焦于對免疫細胞的趨化作用，招募特定免疫細胞到炎癥或腫瘤部位，影響免疫應(yīng)答過程，在腫瘤免疫、自身免疫病等領(lǐng)域研究價值高。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于體外實驗環(huán)境下CXCL9表達水平的動態(tài)監(jiān)測，如炎癥模型構(gòu)建、免疫相關(guān)分子機制研究、藥物干預(yù)效果評估等科研場景，為探索CXCL9在免疫調(diào)控網(wǎng)絡(luò)中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

CMK, Humig, MIG, SCYB9, crg-10, monokine induced by gamma interferon|small inducible cytokine B9
縮寫：

CXCL9
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CXCL9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	102
	Range %	97-107
1:4	Average %	92
	Range %	88-96
1:8	Average %	105
	Range %	99-110

回收率：

The recovery of rat CXCL9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	81-96
EDTA plasma (n=4)	98	92104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.