Rat C-C motif chemokine 6 (CCL6) ELISA kit

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產品詳情

產品描述：

大鼠C-C趨化因子6(CCL6)酶聯免疫試劑盒（CSB-EL027415RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CCL6含量。CCL6屬于趨化因子，能趨化特定免疫細胞到炎癥部位。其研究機制主要圍繞它與受體相互作用，調節免疫細胞遷移和活化。深入研究CCL6對揭示免疫調節機制有意義，也有助于探索相關疾病如炎癥性疾病、腫瘤等的治療靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，可為科研人員研究炎癥性疾病發生機制、免疫細胞趨化行為調控、感染病理模型中的細胞因子網絡變化等課題提供可靠工具，尤其適用于動物實驗模型中趨化因子動態監測、藥物干預效果評估等基礎研究場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ccl6C-C motif chemokine 6 ELISA Kit; Small-inducible cytokine A6 ELISA Kit
縮寫：

CCL6
Uniprot No.：

Q68FP3
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CCL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-98
1:2	Average %	98
	Range %	91-104
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	92-103

回收率：

The recovery of rat CCL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	98	93-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

基因功能參考文獻：
1. The mouse C10 analog gene in the rat, and named it as ccl6, was identified and cloned. PMID: 23212328
亞細胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
數據庫鏈接：

KEGG: rno:287910

STRING: 10116.ENSRNOP00000048296

UniGene: Rn.7857