Rat Apolipoprotein A-IV(APOA4) ELISA kit

Rat Apolipoprotein A-IV(APOA4) ELISA kit

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中文名稱：

大鼠載脂蛋白A-IV(APOA4)酶聯(lián)免疫試劑盒
貨號：

CSB-EL001916RA
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠載脂蛋白A-IV(APOA4)酶聯(lián)免疫試劑盒（CSB-EL001916RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOA4含量。APOA4 是一個重要靶點。它在脂質代謝、心血管疾病等方面有重要作用。其研究機制主要聚焦于它在脂蛋白合成與代謝中的功能，以及它對炎癥反應、氧化應激的調節(jié)等，探索其與疾病發(fā)生發(fā)展關聯(lián)，為相關疾病治療提供思路。試劑盒檢測范圍為9.38 ng/mL-600 ng/mL，適用于肥胖、高脂血癥、動脈粥樣硬化等代謝性疾病模型的機制研究，也可用于評估藥物或飲食干預對脂代謝通路的影響，滿足心血管、內分泌等領域的科研需求本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

Apoa4Apolipoprotein A-IV ELISA kit; Apo-AIV ELISA kit; ApoA-IV ELISA kit; Apolipoprotein A4 ELISA kit
縮寫：

APOA4
Uniprot No.：

P02651
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

9.38 ng/mL-600 ng/mL
靈敏度：

2.34 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat APOA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	93
	Range %	87-97
1:400	Average %	101
	Range %	97-105
1:800	Average %	92
	Range %	88-96
1:1600	Average %	95
	Range %	91-101

回收率：

The recovery of rat APOA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	89	83-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
600	2.567	2.615	2.591	2.454
300	1.934	2.078	2.006	1.869
150	1.324	1.397	1.361	1.224
75	0.831	0.822	0.827	0.690
37.5	0.581	0.574	0.578	0.441
18.75	0.334	0.323	0.329	0.192
9.38	0.261	0.268	0.265	0.128
0	0.136	0.137	0.137

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
基因功能參考文獻：
1. demonstrate a critical role of the PI3K/Akt pathway in apoA-IV's anorectic action in lean rats and suggest a defective PI3K pathway in the ventromedial hypothalamus is responsible for the impaired apoA-IV's anorectic action in the diet-induced obese animals PMID: 29037812
2. These results suggest that the elevated mRNA expression levels of Rbp2 and Apoa4 in the rats with SB contribute to the effective esterification and transport of vitamin A. PMID: 25585692
3. Compared to the rats in the control group, the rats in the HF group had the expression levels of 12 genes with significant changes, Agrp, Ghrl, and Nr3c1, Apoa4, Cntf, Ghr, IL-1beta, Ins1, Lepr,Sort, Adcyap1r1 and Adrb1. PMID: 22715380
4. Nuclear ERalpha is the primary mediator of E2's action on apo A-IV gene expression. PMID: 25051443
5. Data suggest that apoAIV in NTS (nucleus of the solitary tract) or/and peripheral Cck (cholecystokinin) require vagal Cck1r (cholecystokinin receptor 1) signaling to elicit satiation; high-fat diet reduces satiating capacity of these signals. PMID: 24564397
6. Octreotide intervention down-regulated expression of apoB mRNA and apoB48 protein, as well as MTP mRNA and proteins. ApoAIV mRNA was not significantly different between obese and octreotide-treated rats. PMID: 23911221
7. peripheral apo AIV requires an intact CCK system and vagal afferents to activate neurons in the hindbrain to reduce food intake PMID: 23027805
8. hypothalamic apo A-IV level and food intake were inversely related during the normal diurnal cycle as well as in the period of restricted feeding suggesting that hypothalamic apo A-IV is involved in the regulation of daily food intake. PMID: 15059955
9. Chronic consumption of a high fat diet significantly reduced apolipoprotein A-IV (apo A-IV) mRNA levels and response of apo A-IV gene expression to dietary lipids in the hypothalamus. PMID: 15082422
10. apo A-IV released from the intestinal mucosa during lipid absorption stimulates the release of endogenous CCK that activates CCK(1) receptors on vagal afferent nerve terminals initiating feedback inhibition of gastric motility. PMID: 15117731
11. data support a synergistic interaction between apo A-IV and melanocortins that reduces food intake by acting downstream of the arcuate PMID: 16166201
12. that apo A-IV, produced by neuronal cells iin brain, may exert its anorectic action by interacting with catabolic regulatory neuropeptides. PMID: 18577393
13. Intraperitoneally injected Apoa4 inhibited food intake and levels were measured in intestinal lymph. PMID: 19020287
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亞細胞定位：

Secreted.
蛋白家族：

Apolipoprotein A1/A4/E family
組織特異性：

Secreted in plasma.
數(shù)據(jù)庫鏈接：

KEGG: rno:25080

UniGene: Rn.15739

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.