Rat Alpha-fetoprotein,AFP ELISA Kit

產品詳情

文獻引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)酶聯(lián)免疫試劑盒（CSB-E08281r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的AFP含量。AFP即甲胎蛋白，是一種糖蛋白，主要由胎兒肝細胞及卵黃囊合成。在成人，AFP升高可能與肝癌、生殖細胞腫瘤等惡性腫瘤相關。研究機制上，它可作為腫瘤標志物輔助診斷、監(jiān)測病情。其產生機制可能與腫瘤細胞的異常表達有關。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產品可廣泛應用于肝癌動物模型研究、干細胞分化監(jiān)測、組織再生機制探索及腫瘤相關信號通路分析等科研領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

AfpAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
縮寫：

AFP
Uniprot No.：

P02773
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	85
	Range %	81-90
1:40	Average %	89
	Range %	82-94
1:80	Average %	101
	Range %	96-108
1:160	Average %	94
	Range %	89-99

回收率：

The recovery of rat AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	97	93-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.