Rat Acylated Ghrelin (AG) ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠酰基化饑餓素(AG)酶聯免疫試劑盒（CSB-E13167r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AG含量。大鼠酰基化饑餓素（AG）是一種由胃內分泌細胞產生的激素，具有促進食欲和調節能量平衡的作用。其活性形式是通過辛酰化修飾的，能夠激活GHSR受體，影響生長激素釋放、食欲和能量代謝。研究顯示，AG在調節血糖和胰島素抵抗中扮演重要角色，其水平變化與肥胖和糖尿病等代謝性疾病密切相關。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產品適用于動物模型研究，如肥胖、糖尿病或攝食調控機制的探索，也可用于體外藥物篩選或激素分泌相關的基礎研究，為科研人員提供穩定可靠的數據支持，滿足代謝調控、內分泌系統研究等領域的實驗需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

AG
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	87-101
1:2	Average %	86
	Range %	80-92
1:4	Average %	93
	Range %	90-95
1:8	Average %	95
	Range %	92-98

回收率：

The recovery of rat AG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-104
EDTA plasma (n=4)	97	95-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.