Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of rat ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

90

Range %

87-93

1:2

Average %

101

Range %

96-108

1:4

Average %

87

Range %

84-91

1:8

Average %

100

Range %

95-105

The recovery of rat ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

99

95-104

EDTA plasma (n=4)

87

83-90

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.582

2.601

2.592

2.474

500

1.959

1.869

1.914

1.796

250

1.301

1.298

1.300

1.182

125

0.823

0.831

0.827

0.709

62.5

0.498

0.501

0.500

0.382

31.2

0.325

0.336

0.331

0.213

15.6

0.237

0.241

0.239

0.121

0

0.117

0.119

0.118