Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

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文獻引用 (34) 說明書下載

中文名稱：

大鼠8羥基脫氧鳥苷(8-OHdG)酶聯免疫試劑盒
貨號：

CSB-E10526r
規格：

96T/48T
價格：

￥3900/￥2500
促銷：
其他：

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產品描述：

大鼠8羥基脫氧鳥苷(8-OHdG)酶聯免疫試劑盒（CSB-E10526r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的8-OHdG含量。8-OHdG（8-羥基脫氧鳥苷）是DNA中的一種氧化損傷產物，由活性氧自由基攻擊DNA分子中的鳥嘌呤堿基第8位碳原子產生。它是氧化應激和DNA損傷的重要生物標志物，與多種疾病如癌癥、心血管疾病和神經退行性疾病有關。研究8-OHdG有助于了解DNA損傷和修復過程，以及評估疾病風險。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于體外細胞模型、動物實驗中的氧化應激評估，如藥物抗氧化效果驗證、環境污染因子（如重金屬、輻射）的DNA損傷研究，以及腦組織、肝臟等器官的氧化損傷機制分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

8-OHdG
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-98
1:2	Average %	96
	Range %	91-102
1:4	Average %	90
	Range %	85-96
1:8	Average %	96
	Range %	88-99

回收率：

The recovery of rat 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-107
EDTA plasma (n=4)	96	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.890	1.780	1.835	1.682
10	1.198	1.128	1.163	1.010
5	0.756	0.726	0.741	0.588
2.5	0.525	0.495	0.510	0.357
1.25	0.321	0.318	0.320	0.167
0.625	0.272	0.262	0.267	0.114
0.312	0.213	0.209	0.211	0.058
0	0.154	0.152	0.153

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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最新研究進展：

8-OHdG是DNA氧化損傷的標志物，最新研究發現，其與肺癌、肝癌、結直腸癌、卵巢癌等多種癌癥的發生和發展有關。研究人員通過對癌癥患者和對照組進行對比分析，發現8-OHdG的水平在癌癥患者中明顯升高，因此可以作為腫瘤的生物標志物之一。
此外，8-OHdG的水平還可以反映環境暴露和生活方式對人體DNA的影響。最新研究表明，8-OHdG的水平與吸煙、飲酒、肥胖、缺乏鍛煉等不良生活習慣有關。因此，8-OHdG的測量可以用作評估生活方式和環境暴露的生物標志物。

另外，一些研究還表明，8-OHdG可能與一些神經退行性疾病的發生和發展有關，包括帕金森病和阿爾茨海默病。在這些疾病中，氧化應激和DNA氧化損傷可能是致病機制之一，而8-OHdG的測量可以用作評估疾病進展和治療效果的生物標志物。

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.