Rat 6-keto-PGF1α ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠6-酮前列腺素F1α(6-keto-PGF1α)酶聯免疫試劑盒（CSB-E14411r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養上清液樣本中的6-keto-PGF1α含量。6-keto-PGF1α是一種由PGI2代謝生成的非活性產物，作為PGI2生物合成的有用標志物。其研究機制涉及HIF-1α、TXB2、6-Keto-PGF1α和HA的聯合檢測，以反映肝臟微循環障礙狀況，其在慢性乙型肝炎等疾病診斷中具有重要意義。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，常用于研究血管舒縮調節、炎癥反應及血栓形成等生理病理過程；支持心血管疾病模型、藥物干預實驗及炎癥機制等基礎研究，尤其適用于探索氧化應激、內皮損傷或抗血小板藥物藥效評價等領域；通過檢測不同樣本中6 - keto - PGF1α的動態變化，可為血管功能相關科研課題提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

6-keto-PGF1α,6-keto-PGF1 alpha
縮寫：

6-keto-PGF1α
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat 6-keto-PGF1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	88
	Range %	82-95
1:4	Average %	90
	Range %	85-95
1:8	Average %	92
	Range %	84-97

回收率：

The recovery of rat 6-keto-PGF1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-100
EDTA plasma (n=4)	98	93-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.