Rat 5-hydroxyindolacetic acid,5-HIAA ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠5羥基吲哚乙酸(5-HIAA)酶聯(lián)免疫試劑盒（CSB-E13983r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的5-HIAA含量。5-HIAA即5-羥吲哚乙酸，是血清素的主要代謝產(chǎn)物。它在神經(jīng)生物學(xué)研究中是重要指標(biāo)，可反映血清素的代謝水平。其研究機(jī)制主要圍繞血清素能神經(jīng)功能展開，通過檢測體內(nèi)含量變化，輔助探究神經(jīng)系統(tǒng)疾病如抑郁、焦慮等發(fā)病機(jī)制。試劑盒檢測范圍為25 ng/mL-400 ng/mL，常用于神經(jīng)遞質(zhì)代謝研究、神經(jīng)退行性疾病模型評估以及精神類藥物作用機(jī)制探索等領(lǐng)域。適用于實(shí)驗(yàn)室開展神經(jīng)生物學(xué)、藥理學(xué)或毒理學(xué)研究，例如評估藥物干預(yù)對5 - 羥色胺代謝通路的影響，或探究特定病理狀態(tài)下神經(jīng)遞質(zhì)動態(tài)變化等科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

5-HIAA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

25 ng/mL-400 ng/mL
靈敏度：

25 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HIAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-100
1:2	Average %	93
	Range %	88-97
1:4	Average %	98
	Range %	94-100
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of rat 5-HIAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	94	91-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.