Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

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中文名稱：

大鼠5` -核苷酸酶，胞膜(NT5E/CD73)酶聯(lián)免疫試劑盒
貨號：

CSB-E14100r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠5` -核苷酸酶，胞膜(NT5E/CD73)酶聯(lián)免疫試劑盒（CSB-E14100r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的NT5E/CD73含量。NT5E/CD73是一個重要靶點，它在腺苷代謝中起關(guān)鍵作用。其高表達可促進腫瘤微環(huán)境免疫抑制。研究顯示，通過抑制CD73活性阻斷腺苷生成，或能逆轉(zhuǎn)免疫抑制，增強機體抗腫瘤免疫反應(yīng)，當(dāng)前圍繞它的靶向治療研究是熱門方向。試劑盒檢測范圍為0.625 mIU/mL-40 mIU/mL，適用于評估CD73在腫瘤微環(huán)境、神經(jīng)退行性疾病模型或代謝性疾病研究中的表達特征，也可用于藥物干預(yù)實驗中的生物標(biāo)志物檢測。為研究腺苷信號通路及其相關(guān)疾病機制提供可靠的科研工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
縮寫：

NT5E/CD73
Uniprot No.：

P21588
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測范圍：

0.625 mIU/mL-40 mIU/mL
靈敏度：

0.156 mIU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	87
	Range %	82-95
1:4	Average %	90
	Range %	85-97
1:8	Average %	93
	Range %	86-101

回收率：

The recovery of rat CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-101
EDTA plasma (n=4)	91	86-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
40	2.504	2.416	2.460	2.358
20	1.933	1.824	1.879	1.777
10	1.386	1.283	1.335	1.233
5	0.814	0.749	0.782	0.680
2.5	0.424	0.397	0.411	0.309
1.25	0.228	0.213	0.221	0.119
0.625	0.154	0.145	0.150	0.048
0	0.103	0.101	0.102

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Biochemical studies on the effect of prostaglandin inhibitors and willow bark extract on liver cirrhosis induced by acetylsalicylate in rats Doha Mohammad Beltag,Journal of Medical and Life Science,2022

功能：

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor.
蛋白家族：

5'-nucleotidase family
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000015057

UniGene: Rn.40132

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.