Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2) ELISA kit

Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2) ELISA kit

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中文名稱：

大鼠3氧代5α類固醇4脫氫酶2(SRD5A2)酶聯免疫試劑盒
貨號：

CSB-EL022654RA
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠3氧代5α類固醇4脫氫酶2(SRD5A2)酶聯免疫試劑盒（CSB-EL022654RA）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的SRD5A2含量。SRD5A2 即 II 型 5α-還原酶，它能將睪酮轉化為二氫睪酮。該靶點與良性前列腺增生、雄激素性脫發等疾病密切相關。研究機制主要聚焦在研發其抑制劑，以減少二氫睪酮生成，改善相關病癥，相關藥物已在臨床應用。試劑盒檢測范圍為6 pg/mL-1200 pg/mL，適用于探索大鼠模型中SRD5A2的表達水平與雄激素相關生理病理過程的關聯性，例如生殖內分泌機制研究、前列腺組織代謝分析或藥物干預對類固醇合成途徑的影響評估等本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Srd5a2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2
縮寫：

SRD5A2
Uniprot No.：

P31214
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

6 pg/mL-1200 pg/mL
靈敏度：

3 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	94
	Range %	89-99
1:4	Average %	96
	Range %	90-103
1:8	Average %	90
	Range %	87-94

回收率：

The recovery of rat SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-101
EDTA plasma (n=4)	93	87-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1200	0.225	0.217	0.221
300	0.411	0.434	0.423
96	0.651	0.632	0.642
24	1.055	1.017	1.036
6	1.497	1.458	1.478
0	2.359	2.450	2.405

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Effect of Fenugreek Extract on Testosterone Propionate-Induced Benign Prostatic Hyperplasia JY Lee, J Bang, J Kim, KS Baek,International Journal of Molecular Sciences,2025
Effects of red ginseng oil (KGC11o) on testosterone-propionate-induced benign prostatic hyperplasia JY Lee,Journal of Ginseng Research,2021

功能：

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
基因功能參考文獻：
1. Results suggest that in the acute phase after spinal cord injury, the increased expression of 18kDa translocator protein and 5alpha-reductase may represent a protective endogenous response against tissue injury. PMID: 27150077
2. findings show that, in the adult brain, 5alphaR2 is distributed in critical regions for behavioral regulation, suggesting that the functional role of this isoform is present throughout the entire lifespan of the individual PMID: 22776423
3. Low Srd5a2 expression is associated with benign prostatic hypertrophy. PMID: 23405234
4. Changes in the expression of steroid 5-alpha reductase isoenzymes in the prostate in response to environmental stress. PMID: 23122426
5. Tesosterone concentrations during postnatal sexual differentiation of the rat central nervous system, among other sex-dependent factors, influence brain levels of 5alpha-Reductase isozymes in adulthood PMID: 22131296
6. 5-alpha-reductase type 2 is confined to motoneuronal cells of anterior horns of rat spinal cord, cells that also are known to express high levels of androgen receptor. PMID: 12899683
7. A method for the quantitation of mRNA levels of Srd5a2 isoenzymes has been developed. PMID: 14592534
8. 5 alpha-R2 labeling was observed in dorsal (DH) and ventral horns of gray matter (Gm). and 60% of 5 alpha-R2 immunostaining colocalized with oligodendrocyte, 25% with neuron, and 15% with astrocyte markers. PMID: 15305365
9. regulatory elements and the minimal promoter were mapped to the 485-bp region upstream of the start codon PMID: 15576829
10. A high-dietary fat and caloric intake upregulates prostate 5alpha-reductase-2 gene expression, and stimulates prostate growth in neonatal, but not adult r PMID: 16425201
11. great sexual dimorphism in the central nervous system (CNS) with respect to both 5alpha-reductase isozymes, and suggest a crucial role of dihydrotestosterone in the sexual dimorphism of the CNS in the female PMID: 16595696
12. The SRD5A2 mRNA expression in normal rat is 4-fold higher than human prostatic tissue. PMID: 16818707
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亞細胞定位：

Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
蛋白家族：

Steroid 5-alpha reductase family
組織特異性：

Expressed in high levels in the prostate and many other androgen-sensitive tissues.
數據庫鏈接：

KEGG: rno:64677

STRING: 10116.ENSRNOP00000009254

UniGene: Rn.9938

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.