Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

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產品詳情

產品描述：

大鼠α1酸性糖蛋白(α1-AGP)酶聯免疫試劑盒（CSB-E12730r）為競爭法ELISA試劑盒，定量檢測血清、血漿、尿液、組織培養上清液、組織勻漿樣本中的ORM1含量。ORM1即阿片受體μ樣1，是一種重要的藥物靶點。研究發現它參與痛覺調節、情緒調控等生理過程。其機制主要在于與內源性配體結合后，通過一系列信號轉導影響細胞功能，在鎮痛、精神疾病治療等方面有潛在研究價值。試劑盒檢測范圍為3.125 ng/mL-800 ng/mL，具備高靈敏度和特異性，適用于炎癥相關機制研究、藥物干預效果評價或疾病動物模型中的生物標志物分析等科研場景，實驗操作流程標準化，配套試劑完整，可滿足實驗室常規檢測需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Orm1Alpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
縮寫：

ORM1
Uniprot No.：

P02764
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, urine, cell culture supernates, tissue homogenates
檢測范圍：

3.125 ng/mL-800 ng/mL
靈敏度：

3.125 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	85-105
1:2000	Average %	89
	Range %	81-98
1:4000	Average %	90
	Range %	82-97
1:8000	Average %	95
	Range %	83-98

回收率：

The recovery of rat α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	95	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
基因功能參考文獻：
1. fatigue upregulates the level of ORM1, which in turn functions as an anti-fatigue protein to enhance muscle endurance via the CCR5 pathway. PMID: 26740279
2. context-dependent nature of ORM1 angioregulatory function is further demonstrated in the CAM assay where ORM1 stimulates developmental angiogenesis without exerting any inhibitory activity PMID: 22916107
3. alpha-1 acid glycoprotein expression is increased by retinoids at the transcriptional level through two distinct DR1 retinoic acid responsive elements PMID: 15157739
4. Surprisingly, AGP-over-expressing mice were not protected against renal ischemia-reperfusion injury. PMID: 15502707
5. Orm1 gene expression highly induced in cardiomyocytes overexpressing mineralocorticoid receptor and treated with aldosterone. PMID: 17234708
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亞細胞定位：

Secreted.
蛋白家族：

Calycin superfamily, Lipocalin family
數據庫鏈接：

KEGG: rno:24614

STRING: 10116.ENSRNOP00000010454

UniGene: Rn.10295