Rabbit matrix metalloproteinase 9,MMP-9 ELISA Kit

Rabbit matrix metalloproteinase 9,MMP-9 ELISA Kit

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中文名稱：

兔子基質金屬蛋白酶9(MMP-9)酶聯免疫試劑盒
貨號：

CSB-E06933Rb
規格：

96T/48T
價格：

￥4200/￥3000
促銷：
其他：

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產品描述：

兔子基質金屬蛋白酶9(MMP-9)酶聯免疫試劑盒（CSB-E06933Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MMP9含量。MMP9 即基質金屬蛋白酶 9，在多種生理和病理過程中發揮作用。其背景在于它參與細胞外基質的降解與重塑。研究機制上，它可被多種因素激活，能調控腫瘤細胞的侵襲、轉移，也和炎癥等疾病相關，是疾病治療和藥物研發的潛在靶點。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，適用于體外實驗中炎癥機制研究、腫瘤細胞侵襲模型分析、藥物干預效果評估等基礎科研場景，為心血管疾病、纖維化病理、創傷愈合等研究領域提供高效檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
縮寫：

MMP9
Uniprot No.：

P41246
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	99
	Range %	96-102
1:40	Average %	85
	Range %	92-99
1:80	Average %	101
	Range %	98-104
1:160	Average %	85
	Range %	82-89

回收率：

The recovery of rabbit MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	100	96-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1500	0.129	0.127	0.128
750	0.226	0.238	0.232
375	0.379	0.370	0.375
187.5	0.572	0.565	0.569
94	0.799	0.785	0.792
47	0.937	0.987	0.962
23.5	1.148	1.168	1.158
0	1.606	1.634	1.620

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Chemical (Alkali) Burn-Induced Neurotrophic Keratitis Model in New Zealand Rabbit Investigated Using Medical Clinical Readouts and In Vivo Confocal Microscopy (IVCM) M Vohra,Cells,2024
Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits MiljanaBacevic,Archives of Oral Biology,2019

功能：

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
基因功能參考文獻：
1. The present study demonstrated the ability of 30 and 100 ng/ml TIMP3 to attenuate migration and proliferation, and to inhibit the activity of MMP2, MMP9 and TNFalpha secretion of NA SMCs. In conclusion, TIMP3 may be considered a potential therapeutic drug for use in a novel drugeluting stent, to attenuate the progressive dilation of the aortic NA. PMID: 29956789
2. Expression of MMP-9 increased after cerebral aneurysm induction, peaking at week 3, leading to reduced smooth muscle cell number, damaged endothelial cells, and damage to the aneurysm wall elastic layer. PMID: 24534031
3. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases PMID: 26125840
4. Therefore, it was reasonable to speculate that the increased expression of VEGF and MMP-9 in residual hepatic tumor cells and tumor angiogenesis post-embolization would be responsible for the increased metastatic potentiality and invasiveness. PMID: 23392714
5. Performing minimally invasive surgical procedures in the early stages of intracerebral hemorrhage significantly decreases MMP-9. PMID: 23561043
6. Increased expression of MMP-9 in spinal cord follows cervical spondylotic myelopathy. PMID: 22673181
7. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
8. Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
9. In experimental syringomyelia, MMP-9 plays an important role in causing edema in the presyrinx state. PMID: 21092471
10. Tongxinluo can inhibit the expression of MMP-3 and 9 and increase the expression of PPARgamma in atherosclerotic rabbits. PMID: 19778818
11. MMP promoter activation occurs as a function of thoracic aorta wall tension in transgenic mice PMID: 19375723
12. TGF-beta mediated MMP-9 induction may be regulated by the NF-kappaB, Smad3, and JNK pathways, whereas the IL-1beta mediated induction may be regulated by the NF-kappaB and p38 pathways. PMID: 19626678
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
組織特異性：

Osteoclasts.
數據庫鏈接：

KEGG: ocu:100008993

STRING: 9986.ENSOCUP00000025066

UniGene: Ocu.1773

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.