Rabbit matrix metalloproteinase 3,MMP-3 ELISA kit
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中文名稱：兔子基質(zhì)金屬蛋白酶3(MMP-3)酶聯(lián)免疫試劑盒
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貨號：CSB-E09269Rb
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規(guī)格：96T/48T
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價格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：兔子基質(zhì)金屬蛋白酶3(MMP-3)酶聯(lián)免疫試劑盒（CSB-E09269Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP3含量。MMP3 即基質(zhì)金屬蛋白酶 3，它在細(xì)胞外基質(zhì)的降解和重塑等生理過程中發(fā)揮重要作用。其異常表達(dá)與腫瘤侵襲轉(zhuǎn)移、關(guān)節(jié)炎等疾病發(fā)展密切相關(guān)。研究機(jī)制主要聚焦于其對基質(zhì)成分的作用、信號通路的影響等，為相關(guān)疾病治療提供靶點思路。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外培養(yǎng)細(xì)胞、動物模型組織等科研場景中MMP-3的動態(tài)監(jiān)測，為研究結(jié)締組織代謝調(diào)控、炎癥性疾病機(jī)制及藥物干預(yù)效果提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：MMP3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; Transin-1 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Oryctolagus cuniculus (Rabbit)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：0.156 ng/mL-10 ng/mL
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靈敏度：0.039 ng/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Cancer
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 84 Range % 80-94 1:10 Average % 97 Range % 91-105 1:20 Average % 93 Range % 85-97 1:40 Average % 94 Range % 86-99 -
回收率：
The recovery of rabbit MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 89-98 EDTA plasma (n=4) 89 85-96 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 10 2.794 2.694 2.744 2.593 5 2.244 2.144 2.194 2.043 2.5 1.424 1.324 1.374 1.223 1.25 0.854 0.824 0.839 0.688 0.625 0.546 0.536 0.541 0.390 0.312 0.361 0.351 0.356 0.205 0.156 0.230 0.220 0.225 0.074 0 0.152 0.149 0.151 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
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基因功能參考文獻(xiàn)：
- Compromised autophagy may be related to the osteoarthritis progression; JNK and p38 MAPKs up-regulate MMP3 and down-regulate autophagy. PMID: 26873249
- the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
- chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage. PMID: 23520014
- Ulinastatin effectively inhibited the increased expression of MMP-2, MMP-3, and iNOS in degenerated NP cells induced by IL-1beta in vitro. PMID: 22853554
- Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
- Tongxinluo can inhibit the expression of MMP-3 and 9 and increase the expression of PPARgamma in atherosclerotic rabbits. PMID: 19778818
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亞細(xì)胞定位：Secreted, extracellular space, extracellular matrix.
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蛋白家族：Peptidase M10A family
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數(shù)據(jù)庫鏈接：
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