Rabbit matrix metalloproteinase 1,MMP-1 ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔基質金屬蛋白酶1(MMP-1)酶聯免疫試劑盒（CSB-E09744Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP1含量。MMP1 即基質金屬蛋白酶 1，在細胞外基質降解中發揮關鍵作用。異常表達與腫瘤侵襲轉移、關節炎等疾病密切相關。其研究聚焦于解析活性調控機制和與疾病信號通路的關聯，旨在開發針對性藥物以干預相關病理進程。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于基礎醫學研究領域，如探究心血管疾病、關節炎或皮膚纖維化等疾病模型中MMP-1的調控機制，也可用于藥物開發過程中對MMP-1抑制劑的體外效果評估。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

MMP1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit
縮寫：

MMP1
Uniprot No.：

P13943
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	104
	Range %	97-109
1:4	Average %	88
	Range %	82-92
1:8	Average %	98
	Range %	91-102

回收率：

The recovery of rabbit MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-92
EDTA plasma (n=4)	102	92-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X.
基因功能參考文獻：
1. These results suggest that PEP-1-SIRT2 promotes matrix metalloproteinases-induced dedifferentiation via ERK signaling in articular chondrocytes. . PMID: 27697532
2. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
3. Circulating smoke components, including acrolein, contribute to vascular diseases through enhanced MMP-1 and decreased TIMP-3 secretion. PMID: 21742783
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
數據庫鏈接：

KEGG: ocu:100009110

STRING: 9986.ENSOCUP00000015428

UniGene: Ocu.2695