Rabbit luteinizing hormone (LH) ELISA kit

Rabbit luteinizing hormone (LH) ELISA kit

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文獻(xiàn)引用 (1) 說(shuō)明書下載

中文名稱：

兔子黃體生成素（LH）酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E17780Rb
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

兔子黃體生成素（LH）酶聯(lián)免疫試劑盒（CSB-E17780Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LH含量。LH即促黃體生成素，是腺垂體分泌的糖蛋白激素。其生理背景在于參與調(diào)節(jié)生殖系統(tǒng)，對(duì)女性排卵和男性睪酮合成起關(guān)鍵作用。研究機(jī)制上，主要通過(guò)與靶細(xì)胞上受體結(jié)合，激活信號(hào)通路，調(diào)控生殖相關(guān)基因表達(dá)與細(xì)胞功能。試劑盒檢測(cè)范圍為1.56 mIU/mL-100 mIU/mL，該產(chǎn)品可應(yīng)用于兔繁殖生物學(xué)研究、激素水平動(dòng)態(tài)監(jiān)測(cè)、生殖疾病模型評(píng)估或藥物干預(yù)實(shí)驗(yàn)中LH表達(dá)變化的分析，尤其適用于卵巢、垂體等組織局部LH表達(dá)水平的檢測(cè)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

LHBLutropin subunit beta ELISA kit; Lutropin beta chain ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit
縮寫：

LH
Uniprot No.：

Q6IY74
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.56 mIU/mL-100 mIU/mL
靈敏度：

0.39 mIU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	90
	Range %	84-95
1:4	Average %	96
	Range %	89-102
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of rabbit LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-104
EDTA plasma (n=4)	100	94-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
100	2.817	2.839	2.828	2.670
50	2.220	2.281	2.251	2.093
25	1.525	1.579	1.552	1.394
12.5	0.881	0.866	0.874	0.716
6.25	0.557	0.569	0.563	0.405
3.12	0.390	0.384	0.387	0.229
1.56	0.252	0.261	0.257	0.099
0	0.157	0.159	0.158

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Effects of Treatment with Nauclea latifolia Root Decoction on Sexual Behavior and Reproductive Functions in Male Rabbits Alabi QK.et al,J Diet Suppl,2017

功能：

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
基因功能參考文獻(xiàn)：
1. This study determined the cDNA sequences of the luteinizing hormone beta-subunit (LHB) from Japanese White (JW), New Zealand White (NZW), and Dutch-Belted (Dutch) rabbits, and we compared these LHB sequences with those of other mammals. PMID: 17094992
亞細(xì)胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
數(shù)據(jù)庫(kù)鏈接：

KEGG: ocu:100009427

STRING: 9986.ENSOCUP00000025405

UniGene: Ocu.3341

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.