Rabbit follicle-stimulating hormone,FSH ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子促卵泡素(FSH)酶聯(lián)免疫試劑盒（CSB-E06918Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FSH含量。FSH即促卵泡生成素，它在生殖生理中扮演關(guān)鍵角色，調(diào)控卵泡發(fā)育和精子生成。其研究機制圍繞與受體結(jié)合，激活下游信號通路，影響生殖細胞的增殖分化。對FSH深入研究有助于理解生殖內(nèi)分泌疾病，為相關(guān)疾病診斷和治療提供理論支撐。試劑盒檢測范圍為1.56 mIU/mL-100 mIU/mL，該產(chǎn)品適用于科研領(lǐng)域中對兔子生殖內(nèi)分泌系統(tǒng)的機制研究、繁殖調(diào)控實驗評估或藥物對性腺功能影響的體外分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

FSHBFollitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
縮寫：

FSH
Uniprot No.：

Q6IY73
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 mIU/mL-100 mIU/mL
靈敏度：

0.39 mIU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	99
	Range %	91-105
1:4	Average %	103
	Range %	92-110
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
亞細胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
數(shù)據(jù)庫鏈接：

KEGG: ocu:100008946

STRING: 9986.ENSOCUP00000016404

UniGene: Ocu.3174