Rabbit cross linked C-telopeptide of type Ⅱ collagen (CTX-Ⅱ) ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子Ⅱ型膠原C端肽(CTX-Ⅱ)酶聯免疫試劑盒（CSB-E16213Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CTX-Ⅱ含量。CTX-Ⅱ是一種由Ⅱ型膠原降解產生的肽段，是骨關節炎（OA）的潛在生物標志物。其水平與關節損傷密切相關，可用于OA的早期診斷和病情評估。研究顯示，CTX-Ⅱ在OA患者關節液中水平升高，可作為疾病進展及預后的指標。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，常用于骨關節炎、類風濕性關節炎等疾病模型中軟骨退行性病變的機制研究。適用于科研場景中評估實驗動物軟骨代謝狀態、篩選保護軟骨組織的候選化合物，或探究病理條件下膠原降解的動態變化，可為體外實驗提供穩定的數據支持，助力關節疾病相關的基礎研究與藥物開發。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

cross linked C-telopeptide of type 2 collagen (CTX-2),_x000D_ cross linked C-telopeptide of type II collagen (CTX-II)
縮寫：

CTX-Ⅱ
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-103
1:2	Average %	90
	Range %	86-99
1:4	Average %	95
	Range %	90-102
1:8	Average %	92
	Range %	83-99

回收率：

The recovery of rabbit CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	95	89-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.