Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (11) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔血管內(nèi)皮細(xì)胞生長因子(VEGF)酶聯(lián)免疫試劑盒（CSB-E08524Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VEGF含量。VEGF即血管內(nèi)皮生長因子，在腫瘤血管生成、傷口愈合等生理過程中起關(guān)鍵作用。其背景在于它能促進(jìn)內(nèi)皮細(xì)胞增殖、遷移和血管生成。研究機(jī)制方面，主要是與受體結(jié)合，激活下游信號通路，誘導(dǎo)血管生成，是腫瘤治療等領(lǐng)域的重要靶點(diǎn)。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于科研場景下探究血管生成相關(guān)機(jī)制、評估疾病模型中VEGF的動態(tài)變化，或用于藥物研發(fā)中靶向分子效應(yīng)的體外檢測。可為心血管疾病、腫瘤微環(huán)境等領(lǐng)域的基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

VEGF
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-95
1:2	Average %	100
	Range %	91-106
1:4	Average %	94
	Range %	92-105
1:8	Average %	90
	Range %	86-98

回收率：

The recovery of rabbit VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	97	91-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.