Rabbit Tumor necrosis factor α,TNFα ELISA Kit

Rabbit Tumor necrosis factor α,TNFα ELISA Kit

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中文名稱：

兔子腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E06998Rb
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

兔子腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒（CSB-E06998Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的TNF含量。TNF（腫瘤壞死因子）是一種重要的細(xì)胞因子，在免疫調(diào)節(jié)、炎癥反應(yīng)等生理病理過程中發(fā)揮關(guān)鍵作用。研究表明，TNF可激活多條信號(hào)通路，介導(dǎo)細(xì)胞存活、凋亡、炎癥因子釋放等。異常的TNF表達(dá)與多種疾病如類風(fēng)濕關(guān)節(jié)炎等密切相關(guān)，是藥物研發(fā)熱門靶點(diǎn)。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，本試劑盒適用于兔模型在病原體感染、炎癥機(jī)制解析、藥物干預(yù)效果評(píng)估等基礎(chǔ)科研領(lǐng)域的研究需求，為探索TNF-α在免疫應(yīng)答中的動(dòng)態(tài)變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
縮寫：

TNF
Uniprot No.：

P04924
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TNFα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	98
	Range %	92-102
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit TNFα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	92	87-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.905	2.899	2.902	2.789
2500	2.366	2.356	2.361	2.248
1250	1.639	1.604	1.622	1.509
625	0.971	0.939	0.955	0.842
312	0.488	0.456	0.472	0.359
156	0.275	0.259	0.267	0.154
78	0.179	0.171	0.175	0.062
0	0.116	0.110	0.113

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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平均分:

5.0分 - 4 個(gè)評(píng)價(jià)

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他兔

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)：我用CSB-E06998Rb檢測模型組術(shù)前，術(shù)中30min-2h，術(shù)后24h血漿，四參數(shù)模型擬合出來的R2為0.99868（詳見圖片），標(biāo)準(zhǔn)曲線符合預(yù)期。0-2h的TNF-a水平符合升高趨勢，經(jīng)過文獻(xiàn)查詢，該模型需要6h才能檢測出大量升高的TNF-a炎癥因子水平，后續(xù)會(huì)測量6h及以上的炎癥因子水平。課題組師兄推薦，有兔子種屬，且價(jià)格實(shí)惠，測試結(jié)果準(zhǔn)確。

By 陳老師

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他兔

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)：我用CSB-E06998Rb測模型組術(shù)前，術(shù)中30min-2h，術(shù)后24h血漿，標(biāo)準(zhǔn)曲線用四參數(shù)模型擬合出來的R2為0.99783（詳見圖片），標(biāo)準(zhǔn)曲線做了兩列，符合預(yù)期且OD值在0至3.6范圍內(nèi)，且得到的數(shù)據(jù)符合預(yù)期趨勢。該試劑盒測試穩(wěn)定，均能顯色。

By 陳老師

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他兔

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)：我用CSB-E06998Rb測組蛋白注射模型組術(shù)前，術(shù)中30min-2h，術(shù)后24h血漿，標(biāo)準(zhǔn)曲線用四參數(shù)模型擬合出來的R2僅為0.68，而直線擬合出來R2為0.9996（數(shù)據(jù)和擬合曲線方程詳見圖片），標(biāo)準(zhǔn)曲線做了兩列，符合預(yù)期且OD值在0至3.2范圍內(nèi)，且得到的數(shù)據(jù)符合預(yù)期趨勢，術(shù)前測出來約600-800pg/ml。術(shù)中逐漸升高至1400pg/ml，術(shù)后24h降至1000 pg/ml左右，該試劑盒測試穩(wěn)定，做了兩個(gè)復(fù)孔，誤差較小但均能顯色，且兔子個(gè)體差異大，因此我準(zhǔn)備用Fold Change來表示，該數(shù)據(jù)準(zhǔn)備用來發(fā)文章。

By 陳老師

樣品類型：血漿（抗凝劑）水蛭素

樣品信息：其他兔

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)：我用CSB-E06998Rb測正常血漿，組蛋白刺激以及材料和血漿共孵育，標(biāo)準(zhǔn)曲線用四參數(shù)模型擬合出來的R2為0.99814（詳見圖片），標(biāo)準(zhǔn)曲線做了兩列，標(biāo)曲符合預(yù)期且OD值在0至1.6范圍內(nèi)，且得到的數(shù)據(jù)符合預(yù)期趨勢，組蛋白刺激后炎癥因子釋放約為正常組的2倍。該試劑盒測試穩(wěn)定，均能顯色。

By 陳老師

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最新研究進(jìn)展：

腫瘤壞死因子（TNF）是一種細(xì)胞因子，在免疫調(diào)節(jié)、炎癥和細(xì)胞凋亡等生物學(xué)過程中發(fā)揮重要作用。近年來的研究表明，TNF與各種疾病的發(fā)病機(jī)制有關(guān)，例如炎癥性疾病、腫瘤、自身免疫性疾病等。一些新的抗TNF治療藥物也被開發(fā)出來，已經(jīng)用于治療類風(fēng)濕性關(guān)節(jié)炎、克羅恩病等疾病，并獲得了一定的療效。
功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能參考文獻(xiàn)：
1. TUNEL staining was positively correlated with TNF-a protein expression. Our findings suggest that apoptosis can be induced in the vocal fold epithelium after 120min of modal intensity phonation. In contrast, shorter durations of vibration exposure do not result in apoptosis signaling. PMID: 27577014
2. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases. PMID: 26125840
3. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways. PMID: 24070709
4. The TNFalpha-evoked Cl- current. PMID: 24084720
5. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
6. Hypercapnia increased expression of TNFa and decreased expression of NFKB in acute lung injury models. PMID: 21158126
7. In the early stages of myocardial ischemia, bone marrow stem cells are mobilized and home to ischemic myocardium with a concomitant increase in expression of cytokines VEGF and TNFalpha. PMID: 20624659
8. Ammonium perchlorate can increase gene expressions of types I, III collagens, TGF-beta(1) and TNF-alpha in lung of rabbits. PMID: 20937628
9. TNFalpha may either be directly or indirectly involved in vascular damage following an embolic stroke. Moreover, TNFalpha may mediate some of the detrimental effects of tPA on the vascular compartment PMID: 17673188
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亞細(xì)胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
數(shù)據(jù)庫鏈接：

KEGG: ocu:100009088

STRING: 9986.ENSOCUP00000005787

UniGene: Ocu.1971

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.