Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

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中文名稱：

兔子基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E14220Rb
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

兔子基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒（CSB-E14220Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MMP13含量。MMP13即基質(zhì)金屬蛋白酶13，是參與細(xì)胞外基質(zhì)代謝的關(guān)鍵酶。其異常表達(dá)與多種疾病的發(fā)生發(fā)展密切相關(guān)，如骨關(guān)節(jié)炎、腫瘤轉(zhuǎn)移等。研究主要圍繞其在疾病進(jìn)程中的作用機(jī)制，探索以它為靶點(diǎn)開(kāi)發(fā)疾病治療藥物的可能。試劑盒檢測(cè)范圍為93.75 pg/mL-6000 pg/mL，本試劑盒適用于體外實(shí)驗(yàn)研究，可滿足關(guān)節(jié)炎模型構(gòu)建、骨代謝研究、腫瘤微環(huán)境分析等科研場(chǎng)景中MMP - 13的動(dòng)態(tài)監(jiān)測(cè)需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

MMP13Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit
縮寫(xiě)：

MMP13
Uniprot No.：

O62806
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

93.75 pg/mL-6000 pg/mL
靈敏度：

23.44 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	105
	Range %	97-108
1:4	Average %	90
	Range %	85-95
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of rabbit MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-95
EDTA plasma (n=4)	98	90-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.554	2.444	2.499	2.405
3000	1.937	1.976	1.957	1.863
1500	1.409	1.422	1.416	1.322
750	0.815	0.789	0.802	0.708
375	0.422	0.434	0.428	0.334
187.5	0.302	0.289	0.296	0.202
93.75	0.207	0.198	0.203	0.109
0	0.093	0.095	0.094

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

In Vitro and In vivo approaches to evaluate Uncaria tomentosa bark extract loaded FDOFs on Osteoarthritis models JN Sowjanya - 2023 - researchsquare.com,researchsquare,2023
Chondroprotective and antiarthritic effects of daphnetin used in vitro and in vivo osteoarthritis models Xiaohan Zhang, et al,Life Sciences,2019
Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone Li Y. et al,Osteoarthritis Cartilage,2015

功能：

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
基因功能參考文獻(xiàn)：
1. These results suggest that PEP-1-SIRT2 promotes matrix metalloproteinases-induced dedifferentiation via ERK signaling in articular chondrocytes. PMID: 27697532
2. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
3. chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage. PMID: 23520014
4. Leptin can induce MMP-13 and have a synergistic induction effect on NO with TNF-alpha in rabbit articular chondrocytes in vitro. PMID: 19893248
亞細(xì)胞定位：

Secreted, extracellular space, extracellular matrix. Secreted.
蛋白家族：

Peptidase M10A family
數(shù)據(jù)庫(kù)鏈接：

KEGG: ocu:100008685

STRING: 9986.ENSOCUP00000002145

UniGene: Ocu.2263

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.