Rabbit Growth Hormone(GH) ELISA Kit

文獻引用 (1) 說明書下載

產品詳情

產品描述：

兔子生長激素(GH)酶聯免疫試劑盒（CSB-E15021Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 基因編碼生長激素，是人體生長發育的關鍵調節因子。其表達異常與多種生長相關疾病如巨人癥、侏儒癥等有關。研究機制主要聚焦于調控其表達的信號通路，及與其他激素協同作用機制，以探索通過干預 GH1 來治療生長相關疾病的方法。試劑盒檢測范圍為6.25 ng/mL-100 ng/mL，科研中常用于探究營養代謝、生長發育調控及內分泌相關疾病模型的機制研究。適用于科研領域如動物生理學研究、GH相關基因表達調控實驗、藥物或營養干預對激素水平影響的評估，以及兔科動物疾病模型（如垂體功能異常）的機制探索，為實驗室提供高效、可靠的GH定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
縮寫：

GH1
Uniprot No.：

P46407
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-100 ng/mL
靈敏度：

6.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-97
1:2	Average %	95
	Range %	89-100
1:4	Average %	90
	Range %	85-100
1:8	Average %	92
	Range %	89-102

回收率：

The recovery of rabbit GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-110
EDTA plasma (n=4)	95	89-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
亞細胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
數據庫鏈接：

KEGG: ocu:100356068

STRING: 9986.ENSOCUP00000003753