Rabbit Endothelin 1,ET-1 ELISA Kit

Rabbit Endothelin 1,ET-1 ELISA Kit

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中文名稱：

兔子內(nèi)皮素1(ET-1)酶聯(lián)免疫試劑盒
貨號：

CSB-E06951Rb
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

兔子內(nèi)皮素1(ET-1)酶聯(lián)免疫試劑盒（CSB-E06951Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EDN1含量。EDN1即內(nèi)皮素 -1，是一種具有強(qiáng)大而持久血管收縮作用的多肽。在人體生理病理過程中地位重要，與心血管疾病、腫瘤等多種疾病的發(fā)生發(fā)展相關(guān)。研究聚焦于其信號轉(zhuǎn)導(dǎo)機(jī)制、與受體相互作用及在疾病中異常表達(dá)調(diào)控機(jī)制，以助開發(fā)相關(guān)治療策略。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品適用于心血管疾病機(jī)制研究、內(nèi)皮功能評估、藥物干預(yù)效果分析等基礎(chǔ)科研領(lǐng)域，支持在動物模型或離體實(shí)驗(yàn)中定量分析ET-1表達(dá)動態(tài)，為血管生物學(xué)、病理生理學(xué)等研究提供穩(wěn)定可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

EDN1Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
縮寫：

EDN1
Uniprot No.：

P29560
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	96
	Range %	92-104
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	98	90-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.630	2.456	2.543	2.458
200	2.111	2.076	2.094	2.009
100	1.459	1.443	1.451	1.366
50	0.816	0.769	0.793	0.708
25	0.415	0.389	0.402	0.317
12.5	0.218	0.203	0.211	0.126
6.25	0.147	0.149	0.148	0.063
0	0.088	0.082	0.085

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

CT perfusion imaging of cerebral microcirculatory changes following subarachnoid hemorrhage in rabbits: Specific role of endothelin-1 receptor antagonist QuanyangWu.et al,Brain Research Bulletin,2018
Blast Wave Exposure to the Extremities Causes Endothelial Activation and Damage. Spear AM.et al,Shock.,2015

功能：

Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
基因功能參考文獻(xiàn)：
1. Study shows that cerebrovascular muscle cells can express endothelin-1 (ET-1). The production of ET-1 in cerebrovascular muscle cells stimulated by oxyhemoglobin occurs partially through the NF-[kappa]B signal pathway. PMID: 27391329
2. Plasma ET-1 levels of rabbits increased significantly in fluorinated groups compared with those in the control group. PMID: 27941335
3. Dynamic monitoring and comparison of plasma levels of ET, CGRP, NO, and MDA as well as SOD activity revealed that appropriate intervention of these factors may reduce reperfusion injury PMID: 26125755
4. In a saline lavage-induced lung injury model, both circulatory and pulmonary ET-1 levels increased. PMID: 24735960
5. Data suggest elevated levels of endothelin-1, as exhibited in cardiovascular diseases, facilitate development of ventricular arrhythmia by steepening action potential duration restitution and by increasing beat-to-beat variability of repolarization. PMID: 24084217
6. High levels of ET-1 are closely associated with BBB disruption. ET-1 may play an important role in the pathogenesis of secondary brain injury after ICH. PMID: 24034085
7. After subarachnoid hemorrhage, the contractile response to ET-1 was enhanced, and the ET(A) receptor expression was upregulated in the basilar artery. PMID: 21952110
8. After acute pulmonary thromboembolism, thrombolytic and anti-inflammatory treatment could decrease acute lung injury induced by ET-1 and NF-kappaB activation. [endothelin-1; NFKB] PMID: 16207427
9. The increase in myocardial distensibility induced by endothelin-1 is absent in heart failure and is dependent on nitric oxide and prostaglandin release. PMID: 17552879
10. Endothelin-1 enhances nuclear Ca2+ transients in atrial myocytes through Ins(1,4,5)P3-dependent Ca2+ release from perinuclear Ca2+ stores PMID: 18089647
11. The enhancement of gap junction intercellular communication is activated by endothelin-1 via modulating the expression of connexin43, and plays an important role in the pathogenesis of cerebral vasospasm PMID: 18691452
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Endothelin/sarafotoxin family
數(shù)據(jù)庫鏈接：

KEGG: ocu:100009270

STRING: 9986.ENSOCUP00000011526

UniGene: Ocu.5758

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.