Rabbit Aggrecan core protein(ACAN) ELISA kit

Rabbit Aggrecan core protein(ACAN) ELISA kit

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中文名稱：

兔子蛋白聚糖(ACAN)酶聯(lián)免疫試劑盒
貨號：

CSB-E12831Rb
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

兔子蛋白聚糖(ACAN)酶聯(lián)免疫試劑盒（CSB-E12831Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ACAN含量。ACAN即聚集蛋白聚糖，是細胞外基質(zhì)的重要組成部分。它參與維持組織彈性和抗壓性等。研究機制方面，其異常表達與骨關(guān)節(jié)炎、腫瘤等疾病發(fā)生發(fā)展相關(guān)，如調(diào)控細胞增殖、遷移，影響微環(huán)境，目前圍繞它的干預手段是研究熱點。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，本試劑盒適用于軟骨代謝機制、關(guān)節(jié)疾病模型等基礎(chǔ)研究領(lǐng)域。該產(chǎn)品適用于體外評估軟骨細胞分泌功能、病理模型中的基質(zhì)代謝動態(tài)監(jiān)測，或生物材料對軟骨再生的影響等科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

ACAN ELISA Kit; AGC1 ELISA Kit; Aggrecan core protein ELISA Kit; Cartilage-specific proteoglycan core protein ELISA Kit; CSPCP ELISA Kit; Fragment ELISA Kit
縮寫：

ACAN
Uniprot No.：

Q28670
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ACAN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-103
1:2	Average %	89
	Range %	82-94
1:4	Average %	90
	Range %	82-94
1:8	Average %	94
	Range %	87-98

回收率：

The recovery of rabbit ACAN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	103	93-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.020	2.121	2.071	1.944
20	1.902	1.846	1.874	1.747
10	1.570	1.524	1.547	1.420
5	1.238	1.200	1.219	1.092
2.5	0.955	0.934	0.945	0.818
1.25	0.532	0.545	0.539	0.412
0.625	0.315	0.309	0.312	0.185
0	0.128	0.126	0.127

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Pilot-scale expression, purification, and bioactivity of recombinant human TGF-β3 from Escherichia coli MengZhou, et al,European Journal of Pharmaceutical Sciences,2018

功能：

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
蛋白家族：

Aggrecan/versican proteoglycan family
數(shù)據(jù)庫鏈接：

STRING: 9986.ENSOCUP00000024918

UniGene: Ocu.2050

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.