Pig soluble P-selectin,sP-Selectin ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬可溶性P選擇素(sP-Selectin)酶聯免疫試劑盒（CSB-E08106p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的sP-Selectin含量。sP - Selectin 即可溶性 P - 選擇素，它在炎癥反應、血栓形成等病理生理過程中扮演重要角色。研究機制上，它可介導白細胞與內皮細胞、血小板的黏附作用，其水平變化能反映機體炎癥和血管內皮損傷程度，在心血管疾病等研究中備受關注。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于科研領域中對豬心血管疾病模型、炎癥機制或免疫調控相關研究，可通過血清/血漿樣本評估全身性病理狀態，結合細胞培養上清分析體外刺激下的分子釋放規律，或通過組織勻漿探究局部微環境中sP-Selectin的表達水平，為動物疾病模型構建、藥物作用機制探索及生物標志物篩選提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

sP-Selectin
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig sP-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	95
	Range %	92-98
1:4	Average %	87
	Range %	82-90
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of pig sP-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	85	82-89

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.