Pig prolactin (PRL) ELISA Kit

Pig prolactin (PRL) ELISA Kit

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文獻引用 (5) 說明書下載

中文名稱：

豬催乳素(PRL)酶聯免疫試劑盒
貨號：

CSB-E15801p
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

豬催乳素(PRL)酶聯免疫試劑盒（CSB-E15801p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的PRL含量。PRL即泌乳素，是腺垂體分泌的多肽激素。其分泌受下丘腦調節，參與乳腺發育、泌乳啟動與維持等生理過程。近年研究發現它還與腫瘤生長、轉移相關，在腫瘤微環境中影響免疫細胞功能，成為潛在抗癌治療靶點，研究其機制有重要意義。試劑盒檢測范圍為0.78 ng/ml-50 ng/ml.，本試劑盒通過高特異性抗體對目標蛋白進行捕獲與檢測，適用于實驗室環境下針對豬血清或血漿樣本的PRL水平分析，可服務于動物生殖生理機制研究、泌乳相關調控通路探索以及農業養殖中繁殖效率評估等科研方向。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
縮寫：

PRL
Uniprot No.：

P01238
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

0.78 ng/ml-50 ng/ml.
靈敏度：

0.195 ng/ml
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	87-95
1:2	Average %	94
	Range %	89-97
1:4	Average %	99
	Range %	94-102
1:8	Average %	95
	Range %	91-99

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2.677

2.713

2.695

2.495

1.886

1.901

1.894

1.694

12.5

1.365

1.412

1.389

1.189

6.25

1.000

1.032

1.016

0.816

3.12

0.785

0.792

0.789

0.589

1.56

0.546

0.554

0.550

0.350

0.78

0.346

0.324

0.335

0.135

0.199

0.200

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Impacts of hybrid rye and fiber level in the diet of pregnant and early lactation sows on the reproductive performance and fecal microbiota of sows and their piglets L Mazroua, L Lo Verso, J Dallaire,Canadian Journal of Animal Science,2025
Impacts du seigle hybride dans l'alimentation des truies en gestation et en début de lactation sur les performances de reproduction et le microbiote intestinal des truies et leurs porcelets L Mazroua,Thesis&Dissertation,2024
Valine supplementation during late pregnancy in gilts increases colostral protein synthesis through stimulating mTOR signaling pathway in mammary cells Long Che, et al,Amino Acids,2019
Effects of maternal alpha-ketoglutarate supplementation during lactation on the performance of lactating sows and suckling piglets Jiang Q, et al,Archives of Animal Nutrition,2019
Effect of optimizing dietary valine-to-lysine ratio in late gestation or lactation on biochemical indices and performance of lactating primiparous sows KaiguoGao,Animal Feed Science and Technology,2019

功能：

Prolactin acts primarily on the mammary gland by promoting lactation.
基因功能參考文獻：
1. Prolactin and, to a lesser extent progesterone, which increase in early pregnancy, enhance basal and glucose-stimulated insulin secretion in part by increasing glucokinase activity and amplifying cAMP levels. PMID: 14559722
2. iv infusion of prolactin primarily caused coronary, mesenteric, renal, and iliac vasoconstriction PMID: 17463060
3. despite a lack of clear relationship between PRL/C499T polymorphism in 5' UTR of the PRL gene and plasma prolactin cioncentration, gene polymorphism impact on reproductiove performance cannot be excluded PMID: 19675522
亞細胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
數據庫鏈接：

KEGG: ssc:396965

STRING: 9823.ENSSSCP00000001152

UniGene: Ssc.8204

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.