Pig matrix metalloproteinase 9,MMP-9 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬基質(zhì)金屬蛋白酶9(MMP-9)酶聯(lián)免疫試劑盒（CSB-E06798p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP-9含量。MMP-9是一種重要靶點(diǎn)。它在機(jī)體生理與病理過程中發(fā)揮關(guān)鍵作用。在腫瘤中，它能促進(jìn)腫瘤細(xì)胞的侵襲與轉(zhuǎn)移。研究機(jī)制多圍繞其對(duì)細(xì)胞外基質(zhì)的降解、信號(hào)通路的調(diào)控等方面展開，對(duì)其深入研究有助于開發(fā)新的治療策略。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品適用于動(dòng)物模型研究，例如炎癥性疾病機(jī)制探索、創(chuàng)傷修復(fù)過程分析或與MMP - 9相關(guān)的病理狀態(tài)評(píng)估，可為科研人員提供穩(wěn)定可靠的定量數(shù)據(jù)支持，但不可用于臨床診斷或其他非科研用途。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MMP-9
Uniprot No.：

Q2VI03
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	85-106
1:2	Average %	95
	Range %	91-99
1:4	Average %	96
	Range %	88-105
1:8	Average %	95
	Range %	90-102

回收率：

The recovery of pig MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	87-100
EDTA plasma (n=4)	97	93-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.