Pig matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

產品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬基質金屬蛋白酶2/明膠酶A(MMP-2/Gelatinase A)酶聯免疫試劑盒（CSB-E12842p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MMP2/Gelatinase A含量。MMP2/Gelatinase A即基質金屬蛋白酶2，在細胞外基質代謝、組織重塑等生理過程中發揮重要作用。異常表達與腫瘤侵襲轉移、心血管疾病和關節炎等多種疾病相關。研究多聚焦其酶活性調控機制，尋找抑制其活性的靶點，為疾病治療提供新思路。試劑盒檢測范圍為47 ng/mL-3000 ng/mL，適用于科研領域探究豬模型中的組織再生機制、疾病相關分子通路及藥物干預效果評估等方向，為深入解析MMP - 2在生理病理中的功能提供高效檢測方案本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

MMP2/Gelatinase A
Uniprot No.：

F1RF11
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

47 ng/mL-3000 ng/mL
靈敏度：

11.75 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	92-97
1:2	Average %	103
	Range %	99-105
1:4	Average %	93
	Range %	89-98
1:8	Average %	92
	Range %	87-99

回收率：

The recovery of pig MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	90	86-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.