Pig intestinal fatty acid binding protein,iFABP ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬腸脂肪酸結合蛋白(iFABP)酶聯免疫試劑盒（CSB-E13994p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的FABP2含量。FABP2是一個靶點。它在脂肪酸攝取、轉運等過程中發揮重要作用。研究聚焦于其與代謝性疾病關聯機制，如肥胖、糖尿病等。解析其結構和功能，有助于深入了解疾病發病機制，為開發針對性治療藥物提供理論支持。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于腸道炎癥、缺血再灌注損傷等病理機制的科研分析；適用于科研場景中動物模型腸道損傷的動態監測、營養干預對腸道功能影響評估，或藥物研發中腸道保護效果的量化分析；為消化道相關疾病的基礎研究提供標準化檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

FABP2 ELISA Kit; FABPIFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit
縮寫：

FABP2
Uniprot No.：

Q45KW7
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-93
1:2	Average %	100
	Range %	97-103
1:4	Average %	85
	Range %	81-89
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of pig iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	99	93-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
基因功能參考文獻：
1. The full-length cDNA of I-FABP was cloned from intestine and the mRNA was extensively present in various tissues, but I-FABP transcript of approximately 620 bp was more abundant in intestine than in other tissues. PMID: 16529296
2. Plasma I-FABP concentration showed great variation within treatments, and no difference was observed in plasma I-FABP concentrations between the weaned conventionally and unweaned treatments PMID: 18708596
3. Association analyses of the FABP2:g.412T>C with fatty acid composition traits were not significant in simple association. PMID: 19207930
亞細胞定位：

Cytoplasm.
蛋白家族：

Calycin superfamily, Fatty-acid binding protein (FABP) family
數據庫鏈接：

KEGG: ssc:595106

STRING: 9823.ENSSSCP00000025576

UniGene: Ssc.16525