Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit

說(shuō)明書(shū)下載

中文名稱：

豬肝炎病毒細(xì)胞的受體1/腎損傷分子1/KIM1(HAVCR1)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL010144PI
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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豬肝炎病毒細(xì)胞的受體1/腎損傷分子1/KIM1(HAVCR1)酶聯(lián)免疫試劑盒（CSB-EL010144PI）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HAVCR1含量。HAVCR1（甲型肝炎病毒細(xì)胞受體1）是一種跨膜糖蛋白，作為甲型肝炎病毒的受體，其研究揭示了HAV進(jìn)入細(xì)胞的關(guān)鍵機(jī)制。HAVCR1的發(fā)現(xiàn)為甲型肝炎的治療提供了新的靶點(diǎn)，通過(guò)研究其作用機(jī)制，有助于開(kāi)發(fā)新型抗病毒藥物。試劑盒檢測(cè)范圍為0.18 ng/mL-12 ng/mL，本產(chǎn)品適用于科研領(lǐng)域，如建立肝炎或腎損傷動(dòng)物模型后評(píng)估靶蛋白動(dòng)態(tài)變化、分析疾病相關(guān)信號(hào)通路調(diào)控機(jī)制，或用于篩選潛在治療藥物時(shí)監(jiān)測(cè)生物標(biāo)志物水平，為相關(guān)基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

HAVCR1
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.18 ng/mL-12 ng/mL
靈敏度：

0.04 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Microbiology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig HAVCR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-101
1:2	Average %	93
	Range %	82-102
1:4	Average %	95
	Range %	88-99
1:8	Average %	95
	Range %	90-105

回收率：

The recovery of pig HAVCR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-100
EDTA plasma (n=4)	96	90-110

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
12	1.786	1.793	1.790	1.653
6	1.405	1.456	1.431	1.294
3	1.034	1.008	1.021	0.884
1.5	0.669	0.682	0.676	0.539
0.75	0.435	0.448	0.442	0.305
0.375	0.288	0.303	0.296	0.159
0.18	0.185	0.191	0.188	0.051
0	0.135	0.138	0.137

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit

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