Pig cholecystokinin,CCK ELISA Kit

Pig cholecystokinin,CCK ELISA Kit

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中文名稱：

豬膽囊收縮素/腸促胰酶肽(CCK)酶聯(lián)免疫試劑盒
貨號：

CSB-E10131p
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豬膽囊收縮素/腸促胰酶肽(CCK)酶聯(lián)免疫試劑盒（CSB-E10131p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCK含量。CCK即縮膽囊素，是一種胃腸道激素，在神經(jīng)系統(tǒng)也作為神經(jīng)遞質(zhì)存在。其廣泛參與消化調(diào)節(jié)、攝食調(diào)控等生理過程。研究機(jī)制多聚焦于與CCK受體結(jié)合，激活下游信號通路，在消化、神經(jīng)等領(lǐng)域開展研究，助力相關(guān)疾病治療策略探索。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，該產(chǎn)品適用于科研領(lǐng)域中對豬源性樣本CCK表達(dá)水平的定量研究，例如動物消化系統(tǒng)功能評估、神經(jīng)肽作用機(jī)制探索或代謝調(diào)控相關(guān)實驗。通過兼容血清、血漿及多種組織樣本的檢測特性，本試劑盒為研究者提供靈活的實驗方案選擇，助力于豬模型在胃腸生理、神經(jīng)生物學(xué)及代謝疾病機(jī)制等方向的科研工作。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

CCK ELISA Kit; Cholecystokinin ELISA Kit; CCK) [Cleaved into: Cholecystokinin-58 ELISA Kit; CCK58); Cholecystokinin-58 desnonopeptide ELISA Kit; (1-49)-CCK58); Cholecystokinin-39 ELISA Kit; CCK39); Cholecystokinin-33 ELISA Kit; CCK33); Cholecystokinin-25 ELISA Kit; CCK25); Cholecystokinin-18 ELISA Kit; CCK18); Cholecystokinin-12 ELISA Kit; CCK12); Cholecystokinin-8 ELISA Kit; CCK8); Cholecystokinin-7 ELISA Kit; CCK7); Cholecystokinin-5 ELISA Kit; CCK5)] ELISA Kit
縮寫：

CCK
Uniprot No.：

P01356
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-100
1:2	Average %	104
	Range %	101-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	90
	Range %	87-93

回收率：

The recovery of pig CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	92	87-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.179	2.198	2.189	2.031
50	1.698	1.677	1.688	1.530
25	1.136	1.166	1.151	0.993
12.5	0.697	0.658	0.678	0.520
6.25	0.486	0.497	0.492	0.334
3.12	0.383	0.398	0.391	0.233
1.56	0.301	0.321	0.311	0.153
0	0.157	0.159	0.158

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Dietary Branched-Chain Amino Acids Regulate Food Intake Partly through Intestinal and Hypothalamic Amino Acid Receptors in Piglets Tian M,journal of agricultural and food chemistry,2019
Branched chain amino acids stimulate gut satiety hormone cholecystokinin secretion through the activation of the umami taste receptor T1R1/T1R3 using an in vitro porcine jejunum model Min Tian,Food & Function,2019

功能：

This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
基因功能參考文獻(xiàn)：
1. We conclude that only a small fraction of neuronal CCK is nonsulfated. The intracellular distribution of nonsulfated CCK in neurons suggests that they contribute only modestly to the CCK transmitter activity. PMID: 27535680
2. Cardiac expression of pro-cholecystokinin is cell-specific, which differentiates the expression from that of intestinal endocrine cells and cerebral neurons. PMID: 25627687
3. This review explores the role of luminal cholecystokinin on the regulation of exocrine pancreatic secretion in author's studies of neonatal pigs. PMID: 15075451
亞細(xì)胞定位：

Secreted.
蛋白家族：

Gastrin/cholecystokinin family
組織特異性：

Synthesized in both cerebral cortex and duodenal mucosa.
數(shù)據(jù)庫鏈接：

KEGG: ssc:397468

STRING: 9823.ENSSSCP00000012023

UniGene: Ssc.717

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.