Pig calcitonin gene related peptide(CGRP)ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬降鈣素基因相關肽(CGRP)酶聯(lián)免疫試劑盒（CSB-E16438p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CGRP含量。CGRP（降鈣素基因相關肽）是一種具有強大血管舒張活性的神經(jīng)肽。背景上，其在偏頭痛發(fā)病機制中起到重要作用。研究機制方面，它可通過與受體結合，介導神經(jīng)源性炎癥、擴張硬腦膜血管等，引發(fā)偏頭痛相關癥狀，是偏頭痛治療的熱門靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于神經(jīng)生物學、心血管研究、疼痛機制探索等科研領域，尤其適合用于建立動物模型（如偏頭痛或高血壓模型）后評估CGRP動態(tài)變化，或通過組織樣本分析特定器官中CGRP的表達水平。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CGRP
Uniprot No.：

A6P7L6
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-94
1:2	Average %	102
	Range %	97-107
1:4	Average %	99
	Range %	96-102
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of pig CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.