Pig calcitonin gene related peptide(CGRP)ELISA Kit

Pig calcitonin gene related peptide(CGRP)ELISA Kit

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中文名稱：

豬降鈣素基因相關(guān)肽(CGRP)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E16438p
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
促銷(xiāo)：
其他：

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產(chǎn)品描述：

豬降鈣素基因相關(guān)肽(CGRP)酶聯(lián)免疫試劑盒（CSB-E16438p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CGRP含量。CGRP（降鈣素基因相關(guān)肽）是一種具有強(qiáng)大血管舒張活性的神經(jīng)肽。背景上，其在偏頭痛發(fā)病機(jī)制中起到重要作用。研究機(jī)制方面，它可通過(guò)與受體結(jié)合，介導(dǎo)神經(jīng)源性炎癥、擴(kuò)張硬腦膜血管等，引發(fā)偏頭痛相關(guān)癥狀，是偏頭痛治療的熱門(mén)靶點(diǎn)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于神經(jīng)生物學(xué)、心血管研究、疼痛機(jī)制探索等科研領(lǐng)域，尤其適合用于建立動(dòng)物模型（如偏頭痛或高血壓模型）后評(píng)估CGRP動(dòng)態(tài)變化，或通過(guò)組織樣本分析特定器官中CGRP的表達(dá)水平。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

CGRP
Uniprot No.：

A6P7L6
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-94
1:2	Average %	102
	Range %	97-107
1:4	Average %	99
	Range %	96-102
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of pig CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	97	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.028	2.204	2.116	1.932
1000	1.560	1.687	1.624	1.440
500	1.049	1.034	1.042	0.858
250	0.665	0.647	0.656	0.472
125	0.377	0.398	0.388	0.204
62.5	0.312	0.325	0.319	0.135
31.25	0.236	0.241	0.239	0.055
0	0.182	0.185	0.184

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.