Pig brain natriuretic peptide,BNP ELISA Kit

Pig brain natriuretic peptide,BNP ELISA Kit

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文獻(xiàn)引用 (1) 說明書下載

中文名稱：

豬腦鈉素/腦鈉尿肽(BNP)酶聯(lián)免疫試劑盒
貨號：

CSB-E06807p
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豬腦鈉素/腦鈉尿肽(BNP)酶聯(lián)免疫試劑盒（CSB-E06807p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BNP含量。BNP即腦鈉肽，由心室肌細(xì)胞分泌。當(dāng)心室容量負(fù)荷或壓力負(fù)荷增加時(shí)，其分泌增加。它具有利鈉、利尿、擴(kuò)血管等作用，可拮抗腎素-血管緊張素-醛固酮系統(tǒng)。目前圍繞BNP開展多項(xiàng)研究，旨在利用其特性改善心臟功能及應(yīng)對相關(guān)疾病。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于心血管疾病基礎(chǔ)研究、動物疾病模型評估、藥效學(xué)實(shí)驗(yàn)中生物標(biāo)志物動態(tài)監(jiān)測等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

NPPBNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 32 ELISA kit; BNP-32); Brain natriuretic peptide 26 ELISA kit; BNP-26)] ELISA kit
縮寫：

BNP
Uniprot No.：

P07634
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
Range %	86-95
1:2	Average %	94
Range %	89-98
1:4	Average %	85
Range %	80-93
1:8	Average %	98
Range %	92-102

回收率：

The recovery of pig BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-102
EDTA plasma (n=4)	88	84-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.551	2.501	2.526	2.392
1000	2.003	1.961	1.982	1.848
500	1.271	1.212	1.242	1.108
250	0.742	0.719	0.731	0.597
125	0.407	0.435	0.421	0.287
62.5	0.326	0.303	0.315	0.181
31.25	0.227	0.214	0.221	0.087
0	0.139	0.128	0.134

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Endovascular hypothermia improves post-resuscitation myocardial dysfunction by increasing mitochondrial biogenesis in a pig model of cardiac arrest Liu Y, et al,Cryobiology,2019

功能：

Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; Plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. In vivo, induces vasodilation.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.
基因功能參考文獻(xiàn)：
1. CNP and BNP serve as oocyte maturation inhibitor to inhibit oocyte maturation in mammals PMID: 24912131
2. ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. The cardiac gene expression does not explain plasma concentrations. PMID: 23539511
3. Up-regulation of BNP and a reduction in a ratio of sarcoplasmic reticulum calcium ATPase 2a in failing myocardium are observed in a porcine model of tachycardia. PMID: 20880599
4. serum levels predict mortality in chest pain patients in Argentina PMID: 21958326
5. Data show that all five molecules, BNP, ICAM-1, TNF-alpha, VCAM-1 and IL-6, quickly and reliably signaled adverse interactions. PMID: 21471537
6. identification and characterization of a novel alternatively spliced variant of porcine and human NPPB resulting from exon 2 skipping PMID: 20660094
7. Arial natriuretic factor (ANF) and N-terminal proANF may be better suited than brain natriuretic peptide as markers of cardiac preload during the development and treatment of acute heart failure. PMID: 19361150
8. Regional differences in BNP and CNP expression were observed in myocardium with sustained left ventricular dyssynchronous contraction. PMID: 19540894
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亞細(xì)胞定位：

Secreted.; [Brain natriuretic peptide 32]: Secreted.
蛋白家族：

Natriuretic peptide family
組織特異性：

Heart atrium.; [Brain natriuretic peptide 32]: Brain (at protein level).; [Brain natriuretic peptide 26]: Brain (at protein level).
數(shù)據(jù)庫鏈接：

KEGG: ssc:396844

STRING: 9823.ENSSSCP00000003722

UniGene: Ssc.629

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.