Pig apolipoprotein A1 (Apo-A1) ELISA Kit
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中文名稱:豬載脂蛋白A1(Apo-A1)酶聯免疫試劑盒
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貨號:CSB-E06834p
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:豬載脂蛋白A1(Apo-A1)酶聯免疫試劑盒(CSB-E06834p)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是一種位于細胞膜外的分泌蛋白,是高密度脂蛋白(HDL)的主要結構載脂蛋白,在逆向膽固醇轉運途徑中起關鍵作用。其與細胞表面受體SR-BI相互作用,促進膽固醇從組織到肝臟的逆向運輸,具有抗炎、抗動脈粥樣硬化等作用。研究顯示,APOA1與病毒感染、動脈粥樣硬化、糖尿病、神經系統疾病、腫瘤等疾病有關。試劑盒檢測范圍為80 μg/mL-2000 μg/mL,適用于豬脂代謝機制研究、心血管疾病動物模型評估、營養干預對脂蛋白代謝的影響分析等科研領域,為豬相關代謝性疾病的基礎研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:APOA1Apolipoprotein A-I ELISA Kit; Apo-AI ELISA Kit; ApoA-I ELISA Kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA Kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Sus scrofa (Pig)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:80 μg/mL-2000 μg/mL
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靈敏度:50 μg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cardiovascular
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 89-103 1:2 Average % 90 Range % 85-103 1:4 Average % 93 Range % 88-100 1:8 Average % 89 Range % 82-97 -
回收率:
The recovery of pig Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 96-104 EDTA plasma (n=4) 90 86-95 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μg/ml OD1 OD2 Average 2000 0.342 0.348 0.345 800 0.533 0.483 0.508 480 0.682 0.657 0.670 200 1.021 0.987 1.004 80 1.267 1.325 1.296 0 1.903 1.954 1.929 -
數據處理:
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貨期:3-5 working days
相關產品
靶點詳情
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功能:Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
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基因功能參考文獻:
- Alterations in the Apo A-I pattern is a good indicator of the presence and severity of infectious diseases in the pig.Lower overall amounts of Apo A-I were observed in Salmonella typhimurium and Escherichia coli infections. PMID: 25377528
- blood level decreases during experimentally induced acute-phase processes in pigs PMID: 15845530
- down-regulation of apolipoprotein A-I and A-IV messages in the liver may be mediated by interleukin 6 and tumor necrosis factor-alpha PMID: 15878672
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亞細胞定位:Secreted.
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蛋白家族:Apolipoprotein A1/A4/E family
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組織特異性:Major protein of plasma HDL, also found in chylomicrons. Synthesized predominantly in the intestine and the liver.
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數據庫鏈接:
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